Kashiba H, Senba E, Ueda Y, Tohyama M
Department of Physiology, Kansai College of Acupuncture Medicine, Osaka, Japan.
Peptides. 1990 May-Jun;11(3):491-6. doi: 10.1016/0196-9781(90)90048-a.
Calcitonin gene-related peptide (CGRP)-containing sensory neurons projecting to viscera or skin were detected by immunocytochemistry combined with fluorescent tracer in the dorsal root ganglia (Th9-10) of rats 5-6 weeks old treated neonatally with capsaicin. The number of CGRP-like immunoreactive (IR) cells were reduced by 50-60% with capsaicin treatment. Visceral CGRP-IR sensory neurons were shown to be more sensitive than cutaneous ones, which was also supported by the fact that CGRP-IR fibers in the stomach were completely diminished while epidermal CGRP-IR fibers were spared.
采用免疫细胞化学结合荧光示踪法，在新生期用辣椒素处理的5至6周龄大鼠的背根神经节（Th9 - 10）中，检测投射至内脏或皮肤的含降钙素基因相关肽（CGRP）的感觉神经元。辣椒素处理使CGRP样免疫反应性（IR）细胞数量减少了50 - 60%。内脏CGRP - IR感觉神经元比皮肤感觉神经元更敏感，这一点也得到以下事实的支持：胃中的CGRP - IR纤维完全消失，而表皮CGRP - IR纤维则得以保留。
