Kovanen P T, Basu S K, Goldstein J L, Brown M S
Endocrinology. 1979 Mar;104(3):610-6. doi: 10.1210/endo-104-3-610.
Low density lipoprotein (LDL)-binding activity was measured in whole homogenates and membranes prepared from fresh bovine adrenal cortex by an ultracentrifugation assay. The binding site for 125I-labeled LDL in isolated membranes shared the properties of the LDL receptor previously demonstrated in intact monolayers of cultured bovine adrenocortical cells. The amount of high affinity [125I]iodo-LDL-binding activity in the adrenal cortex was 6- to 12-fold higher than in the medulla of the same glands. Large amounts of high affinity [125I]iodo-LDL-binding activity were also present in the ovarian corpus luteum but not in the ovarian interstitium. Lesser amounts of high affinity binding activity were observed in 14 other bovine tissues. These results lend support to the concept that cells in the bovine adrenal cortex can obtain cholesterol for steroid hormone synthesis through the receptor-mediated uptake of plasma LDL.
通过超速离心测定法，对新鲜牛肾上腺皮质制备的全匀浆和膜中的低密度脂蛋白（LDL）结合活性进行了测量。分离膜中125I标记的LDL的结合位点具有先前在培养的牛肾上腺皮质细胞完整单层中所证明的LDL受体的特性。肾上腺皮质中高亲和力[125I]碘-LDL结合活性的量比同一腺体的髓质高6至12倍。大量高亲和力[125I]碘-LDL结合活性也存在于卵巢黄体中，但不存在于卵巢间质中。在其他14种牛组织中观察到的高亲和力结合活性较少。这些结果支持了这样一种概念，即牛肾上腺皮质中的细胞可以通过受体介导的血浆LDL摄取来获取用于类固醇激素合成的胆固醇。
