Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg 69120, Germany.
Chem Commun (Camb). 2011 Dec 14;47(46):12536-7. doi: 10.1039/c1cc15476a. Epub 2011 Oct 14.
We report the first example of RNA labeling based on inverse electron-demand Diels-Alder reactions. Both chemically synthesized and enzymatically transcribed RNAs were successfully modified with biotin or a fluorescent label. This approach works efficiently under mild conditions in water and does not require transition metals.
我们报道了首例基于逆电子需求 Diels-Alder 反应的 RNA 标记。通过化学合成和酶转录的 RNA 都可以成功地用生物素或荧光标记进行修饰。该方法在水中的温和条件下高效进行，且不需要过渡金属。
