Nishikawa T, Mikami K, Tamura Y, Yamamoto M, Kumagai A
Endocrinol Jpn. 1979 Feb;26(1):9-17. doi: 10.1507/endocrj1954.26.9.
The responses of the cyclic AMP-generation system and corticosteroids biosynthesis to ACTH and angiotensin II and cholesterol and other lipid contents in adrenal tissues were estimated in the in vitro experiments in 3 cases of Cushing's syndrome due to ACTH-responsive and unresponsive adenomas, one case of Cushing's disease (diffuse hyperplasia), one case of primary aldosteronism and one normal subject. The responses of cAMP accumulation and corticosteroids production to ACTH in in vitro studies were quite in good agreement with the in vivo responses of plasma cortisol by ACTH infusion test. The adenylate cyclase activity decreased and the phosphodiesterase activity increased in the case of hyperplasia and in one case of ACTH-responsive adenoma, whereas the basal cyclic AMP content was slightly more in ACTH-responsive adenoma and maximal in hyperplasia compared with that of the normal adrenal tissue. The characteristic features observed in ACTH-unresponsive adenoma were the largest amount of the basal corticosteroids production and esterified cholesterol content, and the lowest content cAMP. These results indicate that there was not always the consistent correlation between the cAMP-generation system, corticosteroids and aldosterone production, and conversion of cholesterol to pregnenolone by the stimulation of ACTH and angiotensin II in adrenal tumors.
在体外实验中，对3例由促肾上腺皮质激素（ACTH）反应性和无反应性腺瘤引起的库欣综合征患者、1例库欣病（弥漫性增生）患者、1例原发性醛固酮增多症患者以及1名正常受试者的肾上腺组织中，环磷酸腺苷（cAMP）生成系统和皮质类固醇生物合成对ACTH、血管紧张素II以及胆固醇和其他脂质含量的反应进行了评估。体外研究中cAMP积累和皮质类固醇产生对ACTH的反应与ACTH输注试验中血浆皮质醇的体内反应相当一致。在增生病例和1例ACTH反应性腺瘤中，腺苷酸环化酶活性降低，磷酸二酯酶活性增加，而与正常肾上腺组织相比，ACTH反应性腺瘤中的基础cAMP含量略高，增生中最高。在ACTH无反应性腺瘤中观察到的特征是基础皮质类固醇产生量和酯化胆固醇含量最高，而cAMP含量最低。这些结果表明，在肾上腺肿瘤中，cAMP生成系统、皮质类固醇和醛固酮产生以及ACTH和血管紧张素II刺激下胆固醇向孕烯醇酮的转化之间并不总是存在一致的相关性。
