Wageningen UR Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
Bioresour Technol. 2011 Dec;102(24):11204-11. doi: 10.1016/j.biortech.2011.09.090. Epub 2011 Oct 1.
The production of fermentable substrates from barley straw under various process conditions was studied. Pretreatment included chemical pretreatment with dilute-acid followed by enzymatic hydrolysis; the pretreatment conditions were expressed in a combined severity factor, CS, which ranged in the present study from -1.6 to 1.1. Considering the production of fermentable sugars and the release of inhibitory compounds, the optimal pretreatment conditions were 170°C, 0% sulfuric acid and 60 min, corresponding to CS -0.4. Under these conditions, 21.4 g glucose/L, 8.5 g xylose/L, and 0.5 g arabinose/L were produced, while 0.1g HMF/L, 0.4 g furfural/L, 0.0 g levulinic acid/L, 0.0 g formic acid/L, and 2.1g acetic acid/L were released. The ratio of Σ sugars/Σ inhibitors proved to be a good tool for evaluating the suitability of a hydrolysate for fermentation purposes.
研究了在不同工艺条件下从大麦秸秆中生产可发酵底物。预处理包括稀酸化学预处理，然后进行酶水解；预处理条件用综合严重度因子（CS）表示，本研究中的 CS 值范围为-1.6 至 1.1。考虑到可发酵糖的生产和抑制性化合物的释放，最佳预处理条件为 170°C、0%硫酸和 60 分钟，对应的 CS 值为-0.4。在此条件下，可生产 21.4g/L 葡萄糖、8.5g/L 木糖和 0.5g/L 阿拉伯糖，同时释放 0.1g/L 5-羟甲基糠醛、0.4g/L 糠醛、0.0g/L 乙酰丙酸、0.0g/L 甲酸和 2.1g/L 乙酸。Σ 糖/Σ 抑制剂的比值被证明是评估水解物用于发酵目的的适宜性的有效工具。
