Hjelt-Institute, Department of Forensic Medicine, Laboratory of Forensic Biology, PO Box 40 (Kytösuontie 11) FI-00014, University of Helsinki, Helsinki, Finland.
Forensic Sci Int Genet. 2012 Jul;6(4):e99-102. doi: 10.1016/j.fsigen.2011.09.005. Epub 2011 Oct 17.
In this study, the suitability of the Investigator DIPplex insertion/deletion polymorphism (indel) kit for forensic casework was assessed through the genotyping of 151 Finns and 175 Somalis. Allele frequency and heterozygosity (H) of this 30-indel marker set were determined, and forensic efficacy was evaluated through estimation of discrimination power (DP), match probability (MP), typical paternity index (TPI), power of paternity exclusion (PE), and polymorphic information content (PIC). A high level of discrimination power was observed for the marker set in both sample groups (CDP>0.9999). East-west population substructure found previously in uniparental markers within Finland was not evident for this autosomal set (E-W F(ST)=0.003). High exclusion probability and low subdivision together demonstrate that these markers are well-suited for identification of individuals in Finland. However, values for typical paternity index and power of paternity exclusion were low (TPI range Finns=0.750-1.190, PE=0.996; TPI Somalis=0.680-1.090, PE=0.986) in comparison to standard STR sets, and thus indels are not recommended for use in paternity or kinship investigations, except as a supplement to other more powerful tools.
在这项研究中，通过对 151 名芬兰人和 175 名索马里人的基因分型，评估了 Investigator DIPplex 插入/缺失多态性（indel）试剂盒在法医工作中的适用性。确定了这个 30-indel 标记集的等位基因频率和杂合度（H），并通过估计判别能力（DP）、匹配概率（MP）、典型父权指数（TPI）、父权排除概率（PE）和多态信息含量（PIC）来评估法医学效能。在两个样本组中，标记集都表现出很高的判别能力（CDP>0.9999）。在芬兰单倍体标记中先前发现的东西人口结构亚群在这个常染色体标记集中并不明显（E-W F(ST)=0.003）。高排除概率和低细分共同表明，这些标记非常适合在芬兰识别个体。然而，典型父权指数和父权排除概率的值较低（芬兰人 TPI 范围=0.750-1.190，PE=0.996；索马里人 TPI 范围=0.680-1.090，PE=0.986）与标准 STR 集相比，因此除了作为其他更强大工具的补充外，indels 不建议用于亲子关系或亲属关系调查。
