Forensic DNA Center, National Forensic Service, Seoul 158-707, Republic of Korea; Department of Biological Science, Kongju National University, 182 Gongju, Chungnam 314-701, Republic of Korea.
Forensic Sci Int Genet. 2014 Jan;8(1):80-3. doi: 10.1016/j.fsigen.2013.06.013. Epub 2013 Sep 7.
We assessed the applicability of 30 insertion-deletion polymorphisms (INDELs) in forensic use and the level of genetic diversity in South Korea (n=373) using the Investigator DIPplex kit (Qiagen). Allele frequencies, heterozygocities, and forensic efficacy parameters were determined. No deviation from Hardy-Weinberg equilibrium was observed for any of the INDEL markers. A high level of discrimination power was observed (combined power of discrimination: 0.99999999995). The combined match probability value was 2.84 × 10(-11) and the mean typical paternity indices were 0.878. Furthermore, we found one microvariant allele at HLD93 (rs2307570) that has not been reported. We expect that these 30 loci of INDEL markers will be useful for forensic identification and paternity testing in the South Korean population.
我们使用 Investigator DIPplex 试剂盒（Qiagen）评估了 30 个插入缺失多态性（INDEL）在法医学中的适用性和韩国人群中的遗传多样性水平（n=373）。确定了等位基因频率、杂合度和法医效力参数。任何 INDEL 标记都没有偏离 Hardy-Weinberg 平衡。观察到高的鉴别能力（综合鉴别能力：0.99999999995）。联合匹配概率值为 2.84×10(-11)，平均典型父权指数为 0.878。此外，我们在 HLD93(rs2307570) 发现了一个尚未报道的微变异等位基因。我们预计这些 30 个 INDEL 标记位点将有助于韩国人群的法医鉴定和亲子鉴定。
