使用一种新的简化检测方法检测磷脂酶A2以测量细菌酶水平。
Use of a new simplified assay for phospholipase A2 to measure bacterial enzyme levels.
作者信息
Lumb M R, Roseblade C K, Helmig R, Uldbjerg N, Sullivan M H, Elder M G
机构信息
Institute of Obstetrics & Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
出版信息
Clin Chim Acta. 1990 Jul;189(1):39-46. doi: 10.1016/0009-8981(90)90233-i.
During investigation of possible phospholipase A2 (PLA2) production by pathogenic bacteria associated with preterm labour, a rapid and simple assay method was developed which involved few steps and which could be applied easily to large numbers of samples. The principle difference from previously described methods lies in separation of the reaction products by partitioning them between organic and aqueous solvents, rather than by using thin layer chromatography. This enabled us to determine that none of the bacteria studied released PLA2 into the culture medium spontaneously, and that only Escherichia coli contained high levels of PLA2.
在调查与早产相关的病原菌是否可能产生磷脂酶A2（PLA2）的过程中，开发了一种快速简便的检测方法，该方法步骤少，可轻松应用于大量样本。与先前描述的方法的主要区别在于，通过在有机和水性溶剂之间分配反应产物来分离反应产物，而不是使用薄层色谱法。这使我们能够确定所研究的细菌中没有一种会自发地将PLA2释放到培养基中，并且只有大肠杆菌含有高水平的PLA2。
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