Bonney R C
J Endocrinol. 1985 Nov;107(2):183-9. doi: 10.1677/joe.0.1070183.
Phospholipase A2 activity was measured in human endometrium throughout the menstrual cycle using an assay based on the liberation of oleic acid from 1-palmitoyl-2-[14C]oleoyl phosphatidylcholine. The enzyme was shown to be calcium dependent, to have an optimum pH of 8-9 and an apparent Michaelis constant of 110 mumol/l. Enzyme activity was low in early proliferative-phase tissue (6.08 +/- 1.42 (S.E.M.) pmol oleic acid released/mg protein per min) but rose significantly (P less than 0.01) during the late proliferative phase (10.86 +/- 2.79 pmol/mg per min). There was a tenfold increase in activity 2-4 days after ovulation (45.6 +/- 13.6 pmol/mg per min) which thereafter declined to reach values which at menstruation were not significantly different from those of the proliferative phase (4.5 +/- 1.76 pmol/mg per min). The results indicate that phospholipase A2 activity in human endometrium is related to the stage of the menstrual cycle and suggest that arachidonic acid release may be influenced by oestradiol and progesterone.
利用一种基于从1-棕榈酰-2-[14C]油酰磷脂酰胆碱释放油酸的测定方法，在整个月经周期测量人子宫内膜中的磷脂酶A2活性。结果表明该酶依赖钙，最适pH为8 - 9，表观米氏常数为110μmol/l。在增殖早期组织中酶活性较低（每分钟每毫克蛋白质释放6.08±1.42（标准误）pmol油酸），但在增殖后期显著升高（P＜0.01）（每分钟每毫克10.86±2.79 pmol）。排卵后2 - 4天活性增加了10倍（每分钟每毫克45.6±13.6 pmol），此后下降，在月经时达到与增殖期无显著差异的值（每分钟每毫克4.5±1.76 pmol）。结果表明人子宫内膜中的磷脂酶A2活性与月经周期阶段有关，并提示花生四烯酸的释放可能受雌二醇和孕酮影响。
