Microsaic Systems plc, Unit 2, GMS House, Boundary Rd, Woking, Surrey, GU21 5BX, UK.
Rapid Commun Mass Spectrom. 2011 Nov 15;25(21):3281-8. doi: 10.1002/rcm.5230.
A miniature mass spectrometer capable of detecting analytes eluting from a high-performance liquid chromatography (HPLC) system is described and demonstrated for the first time. The entire instrument, including all pumps and the computer, is contained within a single enclosure that may be conveniently accommodated at the base of the HPLC stack. The microspray ion source, vacuum interface, ion guide, and quadrupole ion filter are all microengineered. These components are fabricated in batches using microelectromechanical systems (MEMS) techniques and considered to be consumables. When coupled to a standard HPLC system using an integrated passive split, the limit of detection for reserpine while scanning the full mass range is 5 ng on-column (1 pg of which is passed to the microspray). The mass range is m/z 100-800, and each spectrum is typically acquired at a rate of 1 scan per second.
一种能够检测高效液相色谱（HPLC）系统中洗脱分析物的微型质谱仪被首次描述和演示。整个仪器，包括所有的泵和计算机，都包含在一个单独的外壳中，可以方便地容纳在 HPLC 柱的底部。微喷雾离子源、真空接口、离子导和四极离子过滤器都是微工程化的。这些组件使用微机电系统（MEMS）技术批量制造，并被认为是消耗品。当使用集成的被动分流器将其与标准的 HPLC 系统耦合时，在扫描全质量范围时，利血平的检测限为柱上 5ng（其中 1pg 被传递到微喷雾）。质量范围为 m/z 100-800，每个光谱通常以每秒 1 次扫描的速率获取。
