Dormehl I C, Maree M, Cromarty D, Böckmann H, Jacobs L, van Rensburg E, Kilian J
AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa.
Eur J Nucl Med. 1990;16(8-10):643-7. doi: 10.1007/BF00998163.
The purpose of this study was the correlation of neutrophil kinetics with the pathogenic course of septic shock in the baboon model. Radioactively labelled neutrophils were traced in vivo in normal baboons (n = 6) and in Escherichia coli-infected baboons, which were reinjected with labelled autologous neutrophils either 2 h after the onset of the E. coli infusion (procedure A) (n = 3) or simultaneously with the infusion (procedure B) (n = 3). Cell isolation was done according to a method developed in this laboratory. The cells were labelled with tropolonate In 111, resuspended in 1-2 ml plasma and reinjected. One-minute images were taken every 5th min and then hourly for 4 h with a gamma camera and analysed with a data processor. Time-activity curves were obtained for neutrophil washout from the lungs and neutrophil accumulation in the liver and spleen. These curves were compared for normal baboons and for those treated according to procedures A and B. A significant retention of neutrophils in the lungs of baboons with E. coli-induced septic shock was noted as well as an abnormally slow rate of accumulation in the liver and spleen. It also seems that any lung injury which could be attributed to changes in neutrophil behaviour should be traced back to events during the early exposure of neutrophils to bacterial infection.
本研究的目的是在狒狒模型中探讨中性粒细胞动力学与脓毒性休克病程的相关性。在正常狒狒（n = 6）以及感染大肠杆菌的狒狒体内追踪放射性标记的中性粒细胞，感染大肠杆菌的狒狒在大肠杆菌输注开始后2小时（方法A）（n = 3）或与输注同时（方法B）（n = 3）重新注射标记的自体中性粒细胞。细胞分离按照本实验室开发的方法进行。细胞用铟 - 111托酚酮标记，重悬于1 - 2毫升血浆中并重新注射。每隔5分钟拍摄1分钟图像，然后用γ相机每小时拍摄4小时，并通过数据处理器进行分析。获得了中性粒细胞从肺中清除以及在肝脏和脾脏中积聚的时间 - 活性曲线。对正常狒狒以及按照方法A和B处理的狒狒的这些曲线进行了比较。注意到大肠杆菌诱导的脓毒性休克狒狒的肺中中性粒细胞有显著滞留，以及在肝脏和脾脏中积聚的速率异常缓慢。似乎任何可归因于中性粒细胞行为变化的肺损伤都应追溯到中性粒细胞早期接触细菌感染期间的事件。
