Su An-Ping, Li Zhen-Jun, Tiani Bo-Le, Cao Shuang-Shuang, Zhang Yi, Wang Zhen-Liang, Hu Tao, Shah Ram Babu, Wang Wei-Guo
Department of Hepatobiliopancreatic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Sep;42(5):633-7.
To identify differentially expressed proteins between pancreatic tumor tissues and the adjacent noncancerous tissues by comparative proteomics analysis.
Six pairs pancreatic tumor tissues and the adjacent noncancerous tissues were obtained and selected for two-dimensional polyacrylamide gel electrophoresis (2-DE) analysis. The differentially expressed proteins were identified using the PDQuest 2-D analysis software. After cutting and enzymolysis, the differentially expressed proteins were analysised by ESI-Q-TOF mass spectrometer. The MS/MS data were acquired and searched in the Swiss-Prot database using MASCOT software. Altered expression of representative proteins was validated by immunohistochemical staining.
A total of 97 points were identified as differentially expressed by two-dimensional gel electrophoresis, of which 31 were successfully identified by ESI-Q-TOF mass spectrometer. Among the proteins identified by mass spectrometer, 23 were upregulated and the other 8 were downregulated. These proteins were involved in different functional processes and protein families: glucolysis, hyaluronidase, I, II-phase metabolic enzymes, metalloproteinase, cytoskeleton, Ca2+ metabolism and so on. Immunohistochemical staining revealed an overexpression of HYAL1 and low expression of CYP2C8 and GSTM2 in pancreatic tumor.
The differentially expressed proteins between pancreatic tumor tissues and the adjacent noncancerous tissues can be identified by comparative proteomics approach. The selected proteins will provide a valuable clue and help for further research.
通过比较蛋白质组学分析鉴定胰腺肿瘤组织与相邻非癌组织之间差异表达的蛋白质。
获取6对胰腺肿瘤组织及其相邻的非癌组织，用于二维聚丙烯酰胺凝胶电泳（2-DE）分析。使用PDQuest二维分析软件鉴定差异表达的蛋白质。切胶酶解后，通过电喷雾四极杆飞行时间质谱仪（ESI-Q-TOF）分析差异表达的蛋白质。使用MASCOT软件采集MS/MS数据并在Swiss-Prot数据库中进行检索。通过免疫组织化学染色验证代表性蛋白质的表达变化。
二维凝胶电泳共鉴定出97个差异表达点，其中31个通过ESI-Q-TOF质谱仪成功鉴定。在质谱鉴定的蛋白质中，23个上调，另外8个下调。这些蛋白质涉及不同的功能过程和蛋白质家族：糖酵解、透明质酸酶、I、II期代谢酶、金属蛋白酶、细胞骨架、钙代谢等。免疫组织化学染色显示胰腺肿瘤中HYAL1过表达，CYP2C8和GSTM2低表达。
通过比较蛋白质组学方法可鉴定胰腺肿瘤组织与相邻非癌组织之间差异表达的蛋白质。所筛选出的蛋白质将为进一步研究提供有价值的线索和帮助。
