Lü Xin, Zhang Fanglin, Li Yucheng, Xue Xiaoping, Yin Wen, Xu Zhikai
Department of Microbiology, College of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
Hybridoma (Larchmt). 2011 Oct;30(5):445-50. doi: 10.1089/hyb.2011.0016.
The nucleocapsid protein (NP) of hantavirus has been shown to be highly immunogenic in laboratory animals and humans, and it induces an early and long-lasting humoral immune response during hantavirus infection. In the present study, entire and three partially truncated open reading frames (ORF) of NP genes of Hantaan virus (HTNV) strain 76-118, a prototype of hantavirus, were amplified and then cloned in prokaryotic expression vectors. The recombinant whole NP and truncated NPs were expressed in Escherichia coli. In addition, their antigenic and biological activities were evaluated in an enzyme-linked immunosorbent assay (ELISA) for detection of a series of monoclonal antibodies (MAbs). Results showed that the expressed complete HTNV NP had a similar function as an authentic viral NP. The antigen sites on NP of hantavirus were mainly located in the N-terminus, and the region at 1-37 amino acid might be one important antigenic domain.
汉坦病毒的核衣壳蛋白（NP）已被证明在实验动物和人类中具有高度免疫原性，并且在汉坦病毒感染期间可诱导早期且持久的体液免疫反应。在本研究中，汉坦病毒原型株汉滩病毒（HTNV）76-118株NP基因的完整及三个部分截短的开放阅读框（ORF）被扩增，然后克隆到原核表达载体中。重组的完整NP和截短的NP在大肠杆菌中表达。此外，在用于检测一系列单克隆抗体（MAb）的酶联免疫吸附测定（ELISA）中评估了它们的抗原和生物学活性。结果表明，表达的完整HTNV NP具有与天然病毒NP相似的功能。汉坦病毒NP上的抗原位点主要位于N端，1-37氨基酸区域可能是一个重要的抗原结构域。
