Department of Orthodontics, Institute of Stomatology, Nanjing Medical University, Nanjing, China.
Orthod Craniofac Res. 2011 Nov;14(4):198-205. doi: 10.1111/j.1601-6343.2011.01524.x.
To examine whether periostin-like factor (PLF) stimulation of the expanded palatal suture would accelerate mineral formation of bone.
Expanded palates of 6-week-old male C57BL/6 mice were maintained in an organ culture system, and tissue was either unstimulated or stimulated with PLF or BMP-7 for 8 days. Bone mineral formation was assessed by Raman microspectroscopy analysis and histological examination.
Raman microspectroscopy analysis demonstrated that unstimulated palates maintained their bone mineral concentration within the palatal suture over 8 days (%increase = 11.29 ± 2.34). In comparison, palates exposed to either PLF (%increase = 29.33 ± 1.61, p = 0.012) or BMP-7 (% increase = 25.49 ± 1.09, p = 0.016) formed significantly more bone at the osteogenic fronts of the palatal suture compared with unstimulated samples at day 8. Alkaline phosphatase activity along the bone edges was markedly greater in the PLF and BMP-7 groups compared with that in unstimulated groups at day 0 and day 8. The levels of osteocalcin proteins in the palatal suture tissues of the PLF and BMP-7 groups were significantly higher than those in the unstimulated group.
PLF can increase bone mineral formation within the expanded palatal suture.
研究骨膜蛋白样因子（PLF）刺激扩展的腭中缝是否会加速骨的矿化形成。
将 6 周龄雄性 C57BL/6 小鼠的扩展腭置于器官培养系统中，组织未受刺激或用 PLF 或 BMP-7 刺激 8 天。通过拉曼微光谱分析和组织学检查评估骨矿物质形成。
拉曼微光谱分析表明，未受刺激的腭在 8 天内保持其腭缝内的骨矿物质浓度（%增加= 11.29 ± 2.34）。相比之下，暴露于 PLF（%增加= 29.33 ± 1.61，p = 0.012）或 BMP-7（%增加= 25.49 ± 1.09，p = 0.016）的腭在第 8 天在腭缝的成骨前缘形成明显更多的骨，与未受刺激的样本相比。在第 0 天和第 8 天，PLF 和 BMP-7 组沿骨边缘的碱性磷酸酶活性明显大于未受刺激组。PLF 和 BMP-7 组腭缝组织中的骨钙蛋白水平明显高于未受刺激组。
PLF 可增加扩展的腭中缝内的骨矿物质形成。
