来自产朊假丝酵母的能够在酿酒酵母中自主复制的DNA序列。
DNA sequences from Saccharomycopsis fibuligera capable of autonomous replication in Saccharomyces cerevisiae.
作者信息
Jurský F, Hostinová E
机构信息
Slovak Academy of Sciences, Institute of Molecular Biology, Bratislava.
出版信息
J Basic Microbiol. 1990;30(4):301-4. doi: 10.1002/jobm.3620300419.
A Sau3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast-integrating plasmid YEp24 delta EcoRI and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from these clones were recovered with Escherichia coli, mapped, hybridized with total DNA of S. fibuligera and tested to mitotical stability in Saccharomyces cerevisiae. Experiments suggested the existence of DNA sequences from dimorphic Saccharomycopsis fibuligera with ARS activity in Saccharomyces cerevisiae.
用酵母整合质粒YEp24 delta EcoRI克隆了扣囊复膜孢酵母（Saccharomycopsis fibuligera）总DNA的Sau3AI酶切片段，并在酵母中检测了其自主复制能力（ARS）。从80个克隆中挑选出5个有丝分裂不稳定的酵母转化体，从这些克隆中回收重组质粒，用大肠杆菌进行定位，与扣囊复膜孢酵母的总DNA杂交，并在酿酒酵母（Saccharomyces cerevisiae）中检测其有丝分裂稳定性。实验表明，在酿酒酵母中存在具有ARS活性的双态扣囊复膜孢酵母的DNA序列。
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