School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea.
Biotechnol Lett. 2012 Feb;34(2):303-7. doi: 10.1007/s10529-011-0763-9. Epub 2011 Oct 19.
The ATX1 deletion strain of Saccharomyces cerevisiae is more resistant to Cd(2+) than the wild-type. To investigate the function of Atx1 in Cd(2+) toxicity, we used a metal-binding assay to study the interaction between Atx1 and Cd(2+) in vitro. Using circular dichroism and two-hybrid analyses, we found that Atx1 can bind Cd(2+) specifically and that Cd(2+) binding to Atx1 affects the physical interaction between Atx1 and Ccc2. These results imply that Atx1 delivers Cd(2+) to Ccc2 and that this delivery is, at least in part, responsible for Cd(2+) toxicity in S. cerevisiae.
酿酒酵母 ATX1 缺失菌株比野生型菌株对 Cd(2+)更具抗性。为了研究 Atx1 在 Cd(2+)毒性中的作用，我们使用金属结合测定法研究了 Atx1 与体外 Cd(2+)的相互作用。通过圆二色性和双杂交分析，我们发现 Atx1 可以特异性地结合 Cd(2+)，并且 Cd(2+)与 Atx1 的结合会影响 Atx1 与 Ccc2 之间的物理相互作用。这些结果表明，Atx1 将 Cd(2+)递送给 Ccc2，并且这种递呈至少部分负责 S. cerevisiae 中的 Cd(2+)毒性。
