Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima, 770-8503, Japan.
Biotechnol Lett. 2012 Feb;34(2):213-9. doi: 10.1007/s10529-011-0776-4. Epub 2011 Oct 19.
For proper evaluation of the results of microarray experiments, it is important to understand how the signal intensities of individual probes are determined. Our previous studies revealed that signal intensities of individual probes in the Agilent array system (code G4131F) are largely dependent upon the location of the probes in the mRNA. In the present study, we examined the properties of signal intensities of individual probes in an Affymetrix array system (GeneChip Rat Gene 1.0 ST Array), in which a random primer fused to the T7 promoter sequence is employed. Distinct from the Agilent array system, individual probes used in this Affymetrix array system did not show the probe-location effects, but gave relatively diverse signal intensities. However, the diversities of the signal intensities of these individual probes were not due to experimental error.
为了正确评估微阵列实验的结果，了解单个探针的信号强度是如何确定的非常重要。我们之前的研究表明，Agilent 阵列系统（代码 G4131F）中单个探针的信号强度在很大程度上取决于探针在 mRNA 中的位置。在本研究中，我们研究了 Affymetrix 阵列系统（GeneChip Rat Gene 1.0 ST Array）中单个探针信号强度的特性，在该系统中，随机引物与 T7 启动子序列融合。与 Agilent 阵列系统不同，本 Affymetrix 阵列系统中使用的单个探针没有表现出探针位置效应，但给出了相对多样化的信号强度。然而，这些单个探针的信号强度的多样性并不是由于实验误差造成的。
