Baskys A, Bernstein N K, Barolet A W, Carlen P L
Playfair Neuroscience Unit, Toronto Western Hospital, Canada.
Neurosci Lett. 1990 Apr 20;112(1):76-81. doi: 10.1016/0304-3940(90)90325-4.
Stimulation of N-methyl-D-aspartate (NMDA) or quisqualate (Quis) receptors by submicromolar concentrations of NMDA or Quis but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) reduced post-spike train after hyperpolarizations (AHPs) and blocked the underlying Iahp in dentate granule (DG) neurones in vitro. The NMDA but not Quis action was blocked by the NMDA receptor blocker 2-D,L-aminophosphonovaleric acid (APV). Actions of both NMDA and Quis were abolished by isoquinolinesulphonyl-2-methyl-piperazine dihydrochloride (H-7), an inhibitor of several protein kinases. These data suggest that there is a link between excitatory amino acid receptor activation, the protein kinase system, and neuronal excitability.
亚微摩尔浓度的N-甲基-D-天冬氨酸（NMDA）或喹啉酸（Quis）而非α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）对NMDA或Quis受体的刺激，减少了峰电位后超极化（AHPs），并在体外阻断了齿状颗粒（DG）神经元中潜在的Iahp。NMDA受体阻滞剂2-D，L-氨基磷酸戊酸（APV）可阻断NMDA而非Quis的作用。几种蛋白激酶的抑制剂异喹啉磺酰-2-甲基-哌嗪二盐酸盐（H-7）可消除NMDA和Quis的作用。这些数据表明，兴奋性氨基酸受体激活、蛋白激酶系统和神经元兴奋性之间存在联系。
