分析健康或患病天然牙和牙种植体生物液中的组织蛋白酶 K 水平。
Analysis of cathepsin-K levels in biologic fluids from healthy or diseased natural teeth and dental implants.
机构信息
Department of Periodontology, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
出版信息
Int J Oral Maxillofac Implants. 2011 Sep-Oct;26(5):991-7.
PURPOSE
Cathepsin-K is an enzyme involved in bone metabolism. This feature may make it important both for natural teeth and dental implants. The aims of the present study were to comparatively analyze cathepsin-K levels in gingival crevicular fluid (GCF) and peri-implant sulcus fluid (PISF) and to determine whether GCF and PISF cathepsin-K profiles reflect the clinical periodontal/peri-implant status.
MATERIALS AND METHODS
Clinical parameters (probing depth, Gingival Index, Plaque Index, and bleeding on probing) were recorded, and GCF/PISF samples were obtained from natural teeth (group T) and dental implants (group I), which were divided into groups based on health (clinically healthy, gingivitis/peri-implant mucositis, and periodontitis/peri-implantitis). Cathepsin-K activity was determined with a commercially available cathepsin-K activity assay kit (BioVision).
RESULTS
Sixty natural teeth and 68 dental implants were examined. Teeth with periodontitis (group T-3) showed significantly higher total cathepsin-K activity (10.39 units) than teeth with gingivitis (group T-2, 1.71 units) and healthy teeth (group T-1, 1.90 units). The difference in cathepsin-K activity between groups T-2 and T-1 was not significant. Implants with peri-implantitis (group I-3) had higher total enzyme activity (10.26 units) than healthy implants (group I-1) (3.44 units). Although the difference between clinical parameters was not significant, group I-3 had higher cathepsin-K levels than group I-2 (4.74 units). When natural teeth (T-1, T-2, T-3) were compared to implants (I-1, I-2, I-3), no significant differences were observed for cathepsin-K levels.
CONCLUSION
More cathepsin-K activity was clearly observed with inflammatory periodontal and peri-implant destruction. The highest cathepsin-K levels detected in GCF and PISF samples, obtained from sites with periodontitis and peri-implantitis, suggests the potential involvement of cathespin-K in increased bone metabolism around natural teeth and dental implants.
目的
组织蛋白酶 K 是一种参与骨代谢的酶。这一特性可能使其对天然牙齿和牙种植体都很重要。本研究的目的是比较分析龈沟液（GCF）和种植体周围沟液（PISF）中的组织蛋白酶 K 水平，并确定 GCF 和 PISF 中的组织蛋白酶 K 谱是否反映了临床牙周/种植体状况。
材料和方法
记录临床参数（探诊深度、牙龈指数、菌斑指数和探诊出血），并从天然牙（T 组）和牙种植体（I 组）中获得 GCF/PISF 样本，根据健康状况（临床健康、牙龈炎/种植体黏膜炎和牙周炎/种植体炎）将种植体分为不同组。使用市售的组织蛋白酶 K 活性测定试剂盒（BioVision）测定组织蛋白酶 K 活性。
结果
共检查了 60 颗天然牙和 68 颗牙种植体。患有牙周炎的牙齿（T-3 组）的总组织蛋白酶 K 活性（10.39 单位）明显高于患有牙龈炎的牙齿（T-2 组，1.71 单位）和健康牙齿（T-1 组，1.90 单位）。T-2 组和 T-1 组之间的组织蛋白酶 K 活性差异无统计学意义。患有种植体周围炎的种植体（I-3 组）的总酶活性（10.26 单位）高于健康种植体（I-1 组）（3.44 单位）。尽管临床参数之间的差异无统计学意义，但 I-3 组的组织蛋白酶 K 水平高于 I-2 组（4.74 单位）。当比较天然牙（T-1、T-2、T-3）和牙种植体（I-1、I-2、I-3）时，组织蛋白酶 K 水平无显著差异。
结论
在炎症性牙周和种植体周围破坏时，明显观察到更多的组织蛋白酶 K 活性。在牙周炎和种植体周围炎部位获得的 GCF 和 PISF 样本中检测到的最高组织蛋白酶 K 水平表明，组织蛋白酶 K 可能参与了天然牙和牙种植体周围骨代谢的增加。
Zotero 插件