College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
Poult Sci. 2011 Nov;90(11):2557-64. doi: 10.3382/ps.2010-01238.
Lipid accumulation of avian adipocytes is mainly dependent upon the fatty acid transmembrane uptake process mediated by membrane proteins, such as fatty acid translocase (FAT/CD36), fatty acid transport protein 1, and caveolin-2. To examine the effects of FAT/CD36 on spatial-specific fat deposition, 60 broiler chickens were randomly allocated to 2 groups by sex. Each male or female group contained 2 subgroups (n = 14-15) inoculated by intramuscular injection with chicken FAT/CD36 or BSA (control) immunogens at 34, 49, and 63 d. The subcutaneous and visceral fat deposits were measured, as were levels of plasma triglyceride and free fatty acid. Serum antibody titer was measured by ELISA. The mRNA expression levels of fatty acid transport-related genes in the adipose tissue of the male broilers were investigated to reveal the relationships among various fatty acid transporters. The results showed that active immunization with FAT/CD36 could significantly decrease the visceral fat of the male broilers by up to 40%, but it had no effect on subcutaneous fat stores of male broilers or on either site of fat deposition in female broilers. The concentration of plasma free fatty acids increased in the experimental groups for both male and female broilers. After the FAT/CD36 immunization, very low density lipoprotein receptor mRNA expression was upregulated in both the subcutaneous and visceral fat of male broilers, whereas peroxisome proliferator-activated receptor γ, FAT/CD36, and acyl-CoA binding protein mRNA expression levels were upregulated only in the visceral fat of male broilers. These results indicated a novel role of chicken FAT/CD36 in fat deposition, with sex- and spatial-specific effects.
禽类脂肪细胞的脂类积累主要依赖于脂肪酸跨膜摄取过程，该过程由膜蛋白介导，如脂肪酸易位酶（FAT/CD36）、脂肪酸转运蛋白 1 和窖蛋白-2。为了研究 FAT/CD36 对空间特异性脂肪沉积的影响，将 60 只肉鸡按性别随机分为 2 组。每个雄性或雌性组包含 2 个亚组（n = 14-15），分别在 34、49 和 63 日龄通过肌肉注射接种鸡 FAT/CD36 或 BSA（对照）免疫原。测量皮下和内脏脂肪沉积，以及血浆甘油三酯和游离脂肪酸水平。通过 ELISA 测量血清抗体滴度。通过 RT-PCR 方法检测雄性肉鸡脂肪组织中脂肪酸转运相关基因的 mRNA 表达水平，以揭示各种脂肪酸转运蛋白之间的关系。结果表明，用 FAT/CD36 主动免疫可使雄性肉鸡的内脏脂肪减少 40%，但对雄性肉鸡的皮下脂肪储存或雌性肉鸡任何部位的脂肪沉积均无影响。实验组成年公、母鸡的血浆游离脂肪酸浓度均升高。在 FAT/CD36 免疫后，雄性肉鸡的皮下和内脏脂肪中的极低密度脂蛋白受体 mRNA 表达均上调，而过氧化物酶体增殖物激活受体 γ、FAT/CD36 和酰基辅酶 A 结合蛋白 mRNA 表达水平仅在上皮脂肪中上调。这些结果表明鸡 FAT/CD36 在脂肪沉积中具有新的作用，具有性别和空间特异性。
