Department of Medical Oncology, The First Hospital of Xi'an Jiao Tong University, Xi'an, Shannxi, China.
Scand J Immunol. 2012 Mar;75(3):342-9. doi: 10.1111/j.1365-3083.2011.02657.x.
Previous studies demonstrated that cancer sera contain antibodies, which react with a unique group of autologous cellular antigens called tumour-associated antigens (TAAs). This study determines whether a panel of TAAs would enhance antibody detection and be a useful approach in pancreatic cancer (PC) detection and diagnosis. The panel of TAAs was composed of six TAAs including p53, p16, p62, survivin, Koc and IMP1 full-length recombinant proteins. Enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies against these six TAAs in 23 sera from patients with PC and also 23 sera from normal individuals. Antibody frequency to any individual TAA in PC was variable and ranged from 14.7% to 30.4%. With the successive addition of TAAs to a final total of six antigens, there was a stepwise increase in positive antibody reactions reaching a sensitivity of 60.9% and a specificity of 87.0% in PC. Positive and negative likelihood ratio were 4.685 and 0.449, respectively. Positive and negative predictive values were, respectively, 82.4% and 69.0%. Agreement rate and Kappa value were 73.9% and 0.478, respectively. The data from this study support our previous hypothesis that using a panel of appropriately selected TAAs can enhance autoantibody detection in immunodiagnosis of PC. In 15 PC sera with carbohydrate antigen 19-9 (CA19-9) negative, 6 (40%) were found to have anti-TAA (anti-tumour associated antigens) antibodies. When CA19-9 and anti-TAAs were used together as markers in PC detection, the diagnostic sensitivity could be raised from 60.9% to 69.6%. Anti-TAA and CA19-9 were independent markers, and the simultaneous use of these two markers could raise the sensitivity of PC detection.
先前的研究表明，癌症血清中含有与一组独特的自体细胞抗原（称为肿瘤相关抗原，TAA）反应的抗体。本研究旨在确定一组 TAA 是否会增强抗体检测，并成为胰腺癌（PC）检测和诊断的有用方法。该 TAA 面板由六个 TAA 组成，包括 p53、p16、p62、survivin、Koc 和 IMP1 全长重组蛋白。酶联免疫吸附试验（ELISA）用于检测 23 例 PC 患者血清和 23 例正常人血清中针对这 6 个 TAA 的抗体。PC 中针对单个 TAA 的抗体频率各不相同，范围从 14.7%到 30.4%。随着 TAA 的连续加入，最终达到 6 个抗原，阳性抗体反应逐渐增加，达到 60.9%的敏感性和 87.0%的特异性。阳性和阴性似然比分别为 4.685 和 0.449。阳性和阴性预测值分别为 82.4%和 69.0%。一致性率和 Kappa 值分别为 73.9%和 0.478。本研究的数据支持我们之前的假设，即使用适当选择的 TAA 组可以增强 PC 免疫诊断中的自身抗体检测。在 15 例 CA19-9 阴性的 PC 血清中，有 6 例（40%）发现有抗 TAA（肿瘤相关抗原）抗体。当 CA19-9 和抗 TAA 一起作为 PC 检测的标志物时，诊断敏感性可从 60.9%提高到 69.6%。抗 TAA 和 CA19-9 是独立的标志物，同时使用这两种标志物可以提高 PC 检测的敏感性。
