Department of Radiation Oncology, Cancer Center, Kaohsiung Medical University Hospital, No. 100 Shih-Chuan 1st Road, Kaohsiung, Taiwan.
DNA Cell Biol. 2012 Jan;31(1):43-9. doi: 10.1089/dna.2011.1264. Epub 2011 Oct 19.
Distant metastasis of colorectal cancer (CRC) occurs mainly in the liver and is the major cause of death. This study explored the overexpression of liver metastasis-associated mRNAs in human CRC by using a well-established, weighted enzymatic chip array platform. Analysis of 10 CRC tissue specimens compared with their normal adjacent tissues revealed that ATP2A2, ELAVL4, hTERT, KCTD2, MUC1, OLFM4, S100B, and TM4SF4 genes were upregulated (gene expression ratio of cancer tissue to paired normal tissue was >2) by microarray and bioinformatics analysis. A gene chip including eight candidate genes was constructed to investigate the circulating tumor cells in blood specimens of 103 preoperative CRC patients and further validated by reverse transcriptase-polymerase chain reaction. Liver metastasis was significantly correlated with overexpression of S100B (p=0.001, OR=9.217), TM4SF3 (p=0.011, OR=4.385), and OLFM4 (p=0.015, OR=3.438). These results suggest that S100B, TM4SF3, and OLFM4 overexpression may affect metastatic behavior of tumor cells in Taiwanese CRC patients.
结直肠癌（CRC）的远处转移主要发生在肝脏，是导致死亡的主要原因。本研究通过使用成熟的、加权酶芯片阵列平台，探讨了人 CRC 中与肝转移相关的 mRNA 的过表达。对 10 个 CRC 组织标本与相应正常组织的比较分析显示，ATP2A2、ELAVL4、hTERT、KCTD2、MUC1、OLFM4、S100B 和 TM4SF4 基因通过微阵列和生物信息学分析呈过表达（癌组织与配对正常组织的基因表达比值>2）。构建了一个包含 8 个候选基因的基因芯片，用于研究 103 例术前 CRC 患者血液标本中的循环肿瘤细胞，并通过逆转录-聚合酶链反应进一步验证。肝转移与 S100B（p=0.001，OR=9.217）、TM4SF3（p=0.011，OR=4.385）和 OLFM4（p=0.015，OR=3.438）的过表达显著相关。这些结果表明，S100B、TM4SF3 和 OLFM4 的过表达可能影响台湾 CRC 患者肿瘤细胞的转移行为。
