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钕钇铝石榴石激光照射对根管内根尖牙本质的影响——微渗漏及扫描电镜评估

Nd:YAG laser irradiation effect on apical intracanal dentin - a microleakage and SEM evaluation.

作者信息

Moura-Netto Cacio, Guglielmi Camila de A B, Mello-Moura Anna Carolina Volpi, Palo Renato Miotto, Raggio Daniela Prócida, Caldeira Celso Luiz

机构信息

Department of Endodontics, Dental School, University of São Paulo, Brazil.

出版信息

Braz Dent J. 2011;22(5):377-81. doi: 10.1590/s0103-64402011000500005.

Abstract

The purpose of this in vitro study was to evaluate the effect of neodymium:yttrium-aluminum-garnet (Nd:YAG) laser irradiation on intracanal dentin surface by SEM analysis and its interference in the apical seal of filled canals. After endodontic treatment procedures, 34 maxillary human incisors were randomly assigned to 2 groups. In the negative control group (n=17), no additional treatment was performed and teeth were filled with vertically condensed gutta-percha; in the laser-treated group (n=17), the root canals were irradiated with Nd:YAG laser (1.5 W, 100 mJ, 15 Hz) before filling as described for the control group. Two specimens of each group were prepared for SEM analysis to evaluate the presence and extent of morphological changes and removal of debris; the other specimens were immersed in 0.5% methylene blue dye (pH 7.2) for 24 h for evaluation of the linear dye leakage at the apical third. SEM analysis of the laser-treated group showed dentin fusion and resolidification without smear layer or debris. The Student's t-test showed that the laser-treated group had significantly less leakage in apical third than the control group. Within the limitations of this study, it may be concluded that the morphological changes on the apical intraradicular dentin surface caused by Nd:YAG laser resulted in less linear dye apical leakage.

摘要

本体外研究的目的是通过扫描电子显微镜(SEM)分析评估钕钇铝石榴石(Nd:YAG)激光照射对根管内牙本质表面的影响及其对充填根管根尖封闭的干扰。根管治疗术后,将34颗上颌中切牙随机分为2组。阴性对照组(n = 17)不进行额外处理,用垂直加压牙胶充填牙齿;激光治疗组(n = 17),在充填前按照对照组的方法用Nd:YAG激光(1.5W,100mJ,15Hz)照射根管。每组制备两个标本用于SEM分析,以评估形态学变化的存在和程度以及碎屑的清除情况;其他标本浸泡在0.5%亚甲蓝染料(pH 7.2)中24小时,以评估根尖三分之一处的线性染料渗漏情况。激光治疗组的SEM分析显示牙本质融合和再固化,无玷污层或碎屑。学生t检验显示,激光治疗组根尖三分之一处的渗漏明显少于对照组。在本研究的局限性范围内,可以得出结论,Nd:YAG激光引起的根尖根管内牙本质表面的形态学变化导致线性染料根尖渗漏减少。

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