利用 N-乙基-N-亚硝脲优化斑马鱼基因突变的技术。

Techniques for optimizing the creation of mutations in zebrafish using N-ethyl-N-nitrosourea.

机构信息

Thoren Aquatics, Hazelton, PA, USA.

出版信息

Lab Anim (NY). 2011 Oct 20;40(11):353-61. doi: 10.1038/laban1111-353.

PMID:22012195

Abstract

A safe and successful mutagenesis of zebrafish (Danio rerio) with N-ethyl-N-nitrosourea (ENU) involves balancing several factors. In addition to keeping the fish alive and the humans safe, labor, tank numbers and the process of finding and isolating new mutants should be considered. The author details useful techniques for optimizing zebrafish mutagenesis using ENU.

摘要

用 N-乙基-N-亚硝脲（ENU）对斑马鱼（Danio rerio）进行安全且成功的诱变需要平衡多个因素。除了要让鱼和人都安全外，还应考虑劳动量、鱼缸数量以及寻找和分离新突变体的过程。作者详细介绍了使用 ENU 优化斑马鱼诱变的有用技术。