Li Meiya, Gan Zhibing, Cui Yan, Shi Chunlei, Shi Xianming
MOST-USDA Joint Research Center for Food Safety and Bor Luh Food Safety Center, Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
J Biomed Biotechnol. 2011;2011:731542. doi: 10.1155/2011/731542. Epub 2011 Oct 13.
To elucidate the lutein biosynthesis pathway in the lutein-producing alga, Chlorella protothecoides CS-41, the ζ-carotene desaturase gene (zds) was isolated from Chlorella protothecoides using the approach of rapid amplification of cDNA ends. The full-length cDNA sequence was 2031 bp and contained 1755 bp putative open reading frame which encodes a 584 amino acid deduced polypeptide whose computed molecular weight was 63.7 kDa. Sequence homology research indicated that the nucleotide and putative protein had sequence identities of 72.5% and 69.5% with those of the green alga Chlamydomonas reinhardtii, respectively. Phylogenetic analysis demonstrated that the ZDS from C. protothecoides CS-41 had a closer relationship with those of chlorophyta and higher plants than with those of other species. In addition, we also found that the zds gene expression was upregulated in response to light.
为阐明产叶黄素的藻类原壳小球藻（Chlorella protothecoides）CS - 41中的叶黄素生物合成途径，采用cDNA末端快速扩增方法从原壳小球藻中分离出ζ-胡萝卜素去饱和酶基因（zds）。该全长cDNA序列为2031 bp，包含1755 bp的推定开放阅读框，其编码一个推导的由584个氨基酸组成的多肽，计算分子量为63.7 kDa。序列同源性研究表明，该核苷酸和推定蛋白质与绿藻莱茵衣藻（Chlamydomonas reinhardtii）的序列同一性分别为72.5%和69.5%。系统发育分析表明，原壳小球藻CS - 41的ZDS与绿藻门和高等植物的ZDS关系比与其他物种的更近。此外，我们还发现zds基因表达受光诱导上调。
