Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
Eur Rev Med Pharmacol Sci. 2011 Sep;15(9):992-1002.
Cadmium (Cd) is ubiquitous in the environment and exposure through food and water as well as occupational sources can contribute to a well-defined spectrum of disease. The present study was undertaken to evaluate the role of hesperetin (Hp) in alleviating the Cd induced biochemical changes in rats.
During the experiment, male Wistar rats were injected with Cd 83 mg/kg day) subcutaneously alone or with oral administration of Hp 840 mg/kg day) for 21 days.
In Cd treated rats the levels of plasma lipid peroxidation (LPO) markers: thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LOOH) were significantly increased while the levels of plasma non-enzymatic antioxidants: reduced glutathione (GSH), vitamins C and E were significantly decreased in Cd administered rats. Administration of Hp along with Cd significantly decreased the level of LPO markers with elevation of non-enzymatic antioxidants in plasma. In vitro studies on the effect of Hp on scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH*), 2,2-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS+), superoxide anion (O*-), hydroxyl (OH*) radicals and reducing power also confirmed the free radical scavenging and antioxidant activity of Hp. In addition to that, ascorbic acid, butylated hydroxyl toluene was used as the reference antioxidant radical scavenger compounds. Thus, the observed effects are due to the free radical scavenging and antioxidant potential of Hp. Interestingly, among the different concentrations, tested 50 microM of Hp showed the highest antioxidant and free radical scavenging activities when compared to other concentrations.
The result of these findings provides further evidence to the neutraceutical and pharmaceutical potentials of Hp.
镉（Cd）在环境中普遍存在，通过食物和水以及职业暴露都可能导致明确的疾病谱。本研究旨在评估橙皮素（Hp）在缓解大鼠镉诱导的生化变化中的作用。
在实验过程中，雄性 Wistar 大鼠每天皮下注射 Cd 83mg/kg），或同时口服 Hp 840mg/kg），共 21 天。
在 Cd 处理的大鼠中，血浆脂质过氧化（LPO）标志物：硫代巴比妥酸反应物质（TBARS）和脂质过氧化物（LOOH）的水平显著升高，而血浆非酶抗氧化剂：还原型谷胱甘肽（GSH）、维生素 C 和 E 的水平则显著降低。Hp 与 Cd 同时给药可显著降低 LPO 标志物的水平，并提高血浆中非酶抗氧化剂的水平。体外研究橙皮素对 1,1-二苯基-2-苦基肼（DPPH*）、2,2-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS+）、超氧阴离子（O*-）、羟基（OH*）自由基和还原能力的清除作用也证实了橙皮素的自由基清除和抗氧化活性。此外，抗坏血酸、丁基羟基甲苯被用作参考抗氧化自由基清除剂化合物。因此，观察到的效果是由于 Hp 的自由基清除和抗氧化潜力。有趣的是，在所测试的不同浓度中，50μM 的 Hp 显示出最高的抗氧化和自由基清除活性，优于其他浓度。
这些发现的结果为 Hp 的营养保健品和药物潜力提供了进一步的证据。
