Pari Leelavinothan, Shagirtha Kalist
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar - 608002, Tamilnadu, India.
Exp Toxicol Pathol. 2012 Jul;64(5):513-20. doi: 10.1016/j.etp.2010.11.007. Epub 2010 Dec 4.
The present study was to evaluate the hepatoprotective effect of hesperetin (HTN) on cadmium (Cd) induced hepatotoxicity in male Wistar rats. Administration of Cd (3 mg/kg body weight/day) subcutaneously for 21 days, the levels of hepatic markers such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), gamma glutamyl transferase (GGT) and bilirubin were significantly increased in serum. The levels oxidative stress markers, thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LOOH), conjugated dienes (CD) and protein carbonyl content (PCC) were also significantly increased while the levels of vitamin C, vitamin E, reduced glutathione (GSH), total sulphydryl group (TSH) and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD) were significantly decreased in the liver of Cd intoxicated rats. HTN, a flavanone in citrus fruits, administrated orally along with Cd injection for 21 days, significantly revert back the status of serum hepatic markers, oxidative stress markers and antioxidant markers in the liver tissue to near normal level in a dose dependent manner. HTN at a dose of 40 mg/kg body weight/day exhibits significant (p<0.05) hepatoprotection compared with other two doses (10 and 20 mg/kg body weight/day). The histopathological studies in the liver of rats also supported that HTN (40 mg/kg) markedly reduced the toxicity of Cd and preserved the histoarchitecture of the liver tissue to near normal. Thus, the results suggest that HTN acts as a potent hepatoprotective agent against Cd induced hepatotoxicity in rats.
本研究旨在评估橙皮素（HTN）对镉（Cd）诱导的雄性Wistar大鼠肝毒性的保肝作用。皮下注射Cd（3 mg/kg体重/天）21天，血清中肝标志物如天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）、γ-谷氨酰转移酶（GGT）和胆红素水平显著升高。氧化应激标志物硫代巴比妥酸反应性物质（TBARS）、脂质氢过氧化物（LOOH）、共轭二烯（CD）和蛋白质羰基含量（PCC）水平也显著升高，而镉中毒大鼠肝脏中维生素C、维生素E、还原型谷胱甘肽（GSH）、总巯基（TSH）水平以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽-S-转移酶（GST）、谷胱甘肽还原酶（GR）和葡萄糖-6-磷酸脱氢酶（G6PD）的活性显著降低。HTN是柑橘类水果中的一种黄烷酮，与Cd注射同时口服给药21天，能以剂量依赖的方式使血清肝标志物、氧化应激标志物和肝组织中的抗氧化标志物水平显著恢复至接近正常水平。与其他两个剂量（10和20 mg/kg体重/天）相比，40 mg/kg体重/天的HTN表现出显著（p<0.05）的保肝作用。大鼠肝脏的组织病理学研究也支持HTN（40 mg/kg）显著降低了Cd的毒性，并使肝组织的组织结构保持接近正常。因此，结果表明HTN可作为一种有效的保肝剂，对抗Cd诱导的大鼠肝毒性。
