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一种简单高效的微嫁接方法，用于稳定转化的黄花烟草植物，以研究地上部-根部信号转导。

A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling.

机构信息

Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany.

出版信息

Plant Methods. 2011 Oct 20;7:34. doi: 10.1186/1746-4811-7-34.

DOI:10.1186/1746-4811-7-34
PMID:22014154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3207920/
Abstract

To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and vice versa. Here we describe an efficient micrografting protocol for Nicotiana attenuata, a useful tool for identifying these signals and understanding their functions. Additionally we analyzed transcript accumulation profiles of scions and rootstocks of grafts performed with wild-type and stably transformed N. attenuata. Our results are consistent with the source-to-sink movement of an sRNA silencing signal.

摘要

为了使它们的发育适应环境，植物依赖于从芽到根，或从根到芽传递的特定信号。在这里，我们描述了一种有效的小花烟草(Nicotiana attenuata)微嫁接方案，这是一种识别这些信号并了解其功能的有用工具。此外，我们还分析了用野生型和稳定转化的小花烟草进行嫁接的接穗和砧木的转录物积累谱。我们的结果与一种 sRNA 沉默信号的源到汇的运动一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae0/3207920/cb6ff39e7d59/1746-4811-7-34-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae0/3207920/d3dedc025383/1746-4811-7-34-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae0/3207920/a08b0bb14ea2/1746-4811-7-34-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae0/3207920/cb6ff39e7d59/1746-4811-7-34-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae0/3207920/d3dedc025383/1746-4811-7-34-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae0/3207920/a08b0bb14ea2/1746-4811-7-34-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae0/3207920/cb6ff39e7d59/1746-4811-7-34-3.jpg

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New Phytol. 1997 Nov;137(3):441-452. doi: 10.1046/j.1469-8137.1997.00845.x.
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Isolation and characterization of the threonine deaminase promoter in Nicotiana attenuata.
L中异种移植与同种移植之间的解剖学特征及转录组差异比较
Plants (Basel). 2022 Feb 22;11(5):580. doi: 10.3390/plants11050580.
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Determining the scale at which variation in a single gene changes population yields.确定单个基因变异在何种尺度上改变种群产量。
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Molecular Responses during Plant Grafting and Its Regulation by Auxins, Cytokinins, and Gibberellins.植物嫁接过程中的分子响应及其被生长素、细胞分裂素和赤霉素的调节。
Biomolecules. 2019 Aug 22;9(9):397. doi: 10.3390/biom9090397.
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The Clock Gene TOC1 in Shoots, Not Roots, Determines Fitness of under Drought.在干旱条件下， Shoot 中的 Clock Gene TOC1 而非 Roots 决定了 的适应性。
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