Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA, USA.
J Periodontol. 2012 May;83(5):565-73. doi: 10.1902/jop.2011.110298. Epub 2011 Oct 20.
The objective of this study is to evaluate the potential for periodontal regeneration of a critical-sized defect with the application of recombinant human platelet-derived growth factor (rhPDGF-BB) combined with either a particulate equine or a β-tricalcium phosphate (β-TCP) matrix.
Critical-sized intrabony 2-wall defects were created bilaterally on the distal surface of the second premolar and the mesial surface of the first molar in nine hounds. Twelve defects received rhPDGF-BB/equine treatment, 12 defects received rhPDGF-BB/β-TCP treatment, and the remaining 12 sites served as sham-surgery controls. The animals were sacrificed after a 10-week healing period.
Clinical healing was uneventful without obvious signs of overt gingival inflammation. Histologic and histomorphometric analyses revealed statistically that there were differences among the three groups in terms of new bone formation (P <0.001). The amount of test material for both rhPDGF-BB/equine and rhPDGF-BB/β-TCP groups was comparable, but the amount of newly formed bone was significantly higher (P <0.01) in favor of the rhPDGF-BB/equine group. The amount of new cementum formed for the rhPDGF-BB/equine group (4.8 ± 1.3 mm) was significantly higher (P =0.001) than the sham-surgery control group (1.7 ± 1.9 mm).
Both rhPDGF-BB/equine and rhPDGF-BB/β-TCP have the potential to support the regeneration of the periodontal attachment apparatus.
本研究旨在评估应用重组人血小板衍生生长因子（rhPDGF-BB）联合颗粒状马或β-磷酸三钙（β-TCP）基质治疗牙周临界骨缺损的潜力。
在 9 只猎犬的第二前磨牙远中面和第一磨牙近中面双侧创建临界骨内 2 壁缺损。12 个缺损部位接受 rhPDGF-BB/马处理，12 个缺损部位接受 rhPDGF-BB/β-TCP 处理，其余 12 个部位作为假手术对照。动物在 10 周愈合期后被处死。
临床愈合顺利，无明显明显牙龈炎症迹象。组织学和组织形态计量学分析表明，三组在新骨形成方面存在差异（P<0.001）。rhPDGF-BB/马和 rhPDGF-BB/β-TCP 两组的测试材料量相当，但 rhPDGF-BB/马组的新骨形成量明显更高（P<0.01）。rhPDGF-BB/马组形成的新牙骨质量（4.8±1.3mm）明显高于（P=0.001）假手术对照组（1.7±1.9mm）。
rhPDGF-BB/马和 rhPDGF-BB/β-TCP 均具有支持牙周附着器再生的潜力。
