Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece.
Bioresour Technol. 2012 Jul;115:96-101. doi: 10.1016/j.biortech.2011.09.056. Epub 2011 Oct 1.
Aliphatic esters of cellulose have recently raised the interest on the field of biopolymers. The objective of this work is to develop a methodology for the enzymatic acylation of cellulose with long chain fatty groups in one step. Therefore we designed a system at which fibrous cellulose was enzymatically acylated with vinyl laurate in supercritical carbon dioxide (scCO(2)) and as a result cellulose laurate was formed. The biocatalysts used for this reaction were immobilized lipase Candida antarctica, immobilized esterase from hog liver and the immobilized cutinase Fusarium solani. The ester content of the product varied on the specificity of the biocatalyst used, reaching a maximum of 4.1% after 9h of reaction. In our knowledge, it is the first time where fibrous cellulose is enzymatically acylated by a long chain aliphatic group in one step, without the necessity of any pretreatment.
纤维素的脂族酯最近引起了人们对生物聚合物领域的兴趣。本工作的目的是开发一种在一步中将长链脂肪酸基团酶促酰化纤维素的方法。因此，我们设计了一个系统，在该系统中，纤维状纤维素在超临界二氧化碳（scCO2）中用月桂酸乙烯酯进行酶促酰化，从而形成纤维素月桂酸酯。用于该反应的生物催化剂为固定化脂肪酶 Candida antarctica、猪肝固定化酯酶和固定化角质酶 Fusarium solani。产物的酯含量取决于所用生物催化剂的特异性，在 9 小时的反应后达到最大值 4.1%。据我们所知，这是第一次在无需任何预处理的情况下，通过一步法用长链脂族基团酶促酰化纤维状纤维素。
