尿液患者中血吸虫病抗体的检测作为一种有前途的诊断标志物。
Detection of schistosomiasis antibodies in urine patients as a promising diagnostic maker.
机构信息
Department of Vector and Biomedical Studies, Tropical Medicine Research Institute, National Centre for Research, Khartoum, Sudan.
出版信息
Asian Pac J Trop Med. 2011 Oct;4(10):773-7. doi: 10.1016/S1995-7645(11)60192-2.
OBJECTIVE
To determine secreted antischistosoma antibodies in urine and to discern the epidemiological situation of schistosomiasis in the agricultural field labourers'camps city in the Gezira State-central Sudan.
METHODS
Total of 66 urine and 66 serum paired samples were collected from those who confirmed parasitologically positive and negative with schistosomiasis from the two camps. Samples were tested using ELISA technique to measure and compare the immunoglobulin G (IgG) levels in serum and urine samples of schistosomiasis patients.
RESULTS
The overall prevalence of S. mansoni and S. haematobium was 53.8% and 15.4%, while the intensity were (2.04 GMEC) and (0.9 GMEC) respectively. The relative percentage of positive IgG individulas in urine was 92.40% where as 96.97% in serum. Statistically no significant difference between the IgG levels in serum and urine samples was observed.
CONCLUSIONS
This study shows that the detection of secreted IgG antibodies in urine can substitute serum for diagnosis of schistosomiasis.
目的
在 Gezira 州中心城市的农业营地中，确定尿液中分泌的抗血吸虫抗体，并了解血吸虫病的流行情况。
方法
从两个营地中已确认寄生虫学阳性和阴性的血吸虫病患者中采集了总共 66 份尿液和 66 份血清配对样本。使用 ELISA 技术检测并比较了血吸虫病患者血清和尿液样本中的免疫球蛋白 G（IgG）水平。
结果
曼氏血吸虫和埃及血吸虫的总流行率分别为 53.8%和 15.4%，强度分别为（2.04 GMEC）和（0.9 GMEC）。尿液中阳性 IgG 个体的相对百分比为 92.40%，血清中为 96.97%。统计学上，血清和尿液样本中的 IgG 水平无显著差异。
结论
本研究表明，尿液中分泌的 IgG 抗体的检测可以替代血清用于诊断血吸虫病。
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