苏丹中部杰济拉地区血吸虫病的流行病学及细胞因子谱分析。

Epidemiology of schistosomiasis in Gezira area Central Sudan and analysis of cytokine profiles.

机构信息

Department of Immunology and Biotechnology, Tropical Medicine Research Institute, National Centre for Research, Khartoum, Sudan.

出版信息

Asian Pac J Trop Med. 2013 Feb;6(2):119-25. doi: 10.1016/S1995-7645(13)60006-1.

DOI:10.1016/S1995-7645(13)60006-1

PMID:23339913

Abstract

OBJECTIVE

To determine and compare anti-schistosoma IgG, interleukin-10 (IL-10) and interferon-γ(IFN-γ) levels in the serum of patients and endemic controls and to investigate the epidemiological situation of Al-Hebaika village in the northern part of Gezira Agricultural Irrigation Scheme in 2005.

METHODS

During 2005 survey, serum were collected from 118 villagers. Sixty eight were parasitological positive (patients), and 50 were negative (endemic controls). Indirect ELISA was used to measure and compare the levels of immunoglobulin G (IgG) against Schistsoma mansoni (S. mansoni) soluble worm antigen (SWA) in the patients and endemic control groups from the village and compared with 20 healthy non endemic controls. Sandwich ELISA was also used to measure and compare IL-10 and IFN-γ in the serum of the selected groups.

RESULTS

The overall prevalence of S. haematobium was 20.0% and 0.9% in the first and the second surveys respectively, while the intensity of infection was the same in the two surveys 1.38 [geometric mean egg count (GMFC)]. The overall prevalence of S. mansoni infection was 68.5% and 15.4%, while the intensity of infection was 2.75 (GMEC) and 1.70 (GMEC) in the two surveys respectively. IgG reactivity against SWA showed no significant difference between Schistosoma positive patients and endemic controls. However, there were high significant differences between each of these two groups and the non endemic control group (P= 0,000). Schistosoma patients and exposed controls had significantly higher IL-10 concentration compared with non endemic controls. While endemic controls showed significantly higher IFN-γ concentration than patients (P = 0.000). Also there was very significant difference between IFN-γ levels of each of patients endemic controls and that of the non endemic controls (P = 0.003).

CONCLUSIONS

The study concluded that IFN-γ has a role in the natural resistant to schistosoma mansoni infection. The prevalence and intensity of S. mansoni in the Gezira Irrigation Scheme was greatly reduced. S. haematobium has disappeared from the area.

摘要

目的

确定和比较 2005 年 Gezira 农业灌溉计划北部 Al-Hebaika 村患者和地方对照人群血清中的抗血吸虫 IgG、白细胞介素-10（IL-10）和干扰素-γ（IFN-γ）水平，并调查该地区的流行病学情况。

方法

在 2005 年的调查中，从 118 名村民中采集血清。其中 68 人寄生虫学阳性（患者），50 人阴性（地方对照）。采用间接 ELISA 法检测并比较患者和地方对照人群血清中针对曼氏血吸虫可溶性虫体抗原（SWA）的 IgG 水平，并与 20 名健康非地方对照进行比较。还采用夹心 ELISA 法检测并比较所选人群血清中的 IL-10 和 IFN-γ。

结果

两次调查中，埃及血吸虫的总感染率分别为 20.0%和 0.9%，感染强度相同，均为 1.38（几何均数虫卵计数（GMFC））。曼氏血吸虫感染的总感染率分别为 68.5%和 15.4%，感染强度分别为 2.75（GMEC）和 1.70（GMEC）。SWA 反应性 IgG 在阳性患者和地方对照之间无显著差异。然而，与非地方对照相比，这两个组都有显著的差异（P=0.000）。与非地方对照相比，血吸虫患者和暴露对照的 IL-10 浓度明显更高。而地方对照的 IFN-γ 浓度明显高于患者（P=0.000）。此外，患者、地方对照的 IFN-γ 水平与非地方对照的水平有非常显著的差异（P=0.003）。