Laboratory of Marine Biochemistry, Graduate School of Bioresources, Mie University, 1577 Kurima-machiya, Tsu, Mie 514-8507, Japan.
Mar Environ Res. 2012 May;76:128-37. doi: 10.1016/j.marenvres.2011.09.013. Epub 2011 Oct 5.
In flowering plants, floral homeotic MADS-box genes, which constitute a large multigene family, play important roles in the specification of floral organs as defined by the ABCDE model. In this study, a MADS-box gene, ZjMADS1, was isolated and characterized from the marine angiosperm Zostera japonica. The predicted length of the ZjMADS1 protein was 246 amino acids (AA), and the AA sequence was most similar to those of the SEPALLATA (SEP) subfamily, corresponding to E-function genes. Southern blot analysis suggested the presence of two SEP3-like genes in the Z. japonica genome. ZjMADS1 mRNA levels were extremely high in the spadices, regardless of the developmental stage, compared to other organs from the reproductive and vegetative shoots. These results suggest that the ZjMADS1 gene may be involved in spadix development in Z. japonica and act as an E-function gene in floral organ development in marine angiosperms.
在开花植物中，花同源异型 MADS 框基因构成了一个庞大的多基因家族，在 ABCDE 模型定义的花器官特化中发挥重要作用。本研究从海洋被子植物海菖蒲中分离和鉴定了一个 MADS 框基因 ZjMADS1。ZjMADS1 蛋白的预测长度为 246 个氨基酸 (AA)，AA 序列与 SEPALLATA (SEP) 亚家族最相似，对应于 E 功能基因。Southern blot 分析表明，在海菖蒲基因组中存在两个 SEP3 样基因。ZjMADS1 mRNA 水平在不分发育阶段的佛焰苞中极高，与生殖和营养枝的其他器官相比。这些结果表明，ZjMADS1 基因可能参与海菖蒲佛焰苞的发育，并在海洋被子植物的花器官发育中作为 E 功能基因发挥作用。
