Hosseini Mahmoud, Ghasemzadeh Rahbardar Mahboobeh, Sadeghnia Hamid Reza, Rakhshandeh Hassan
Department of Physiology, Mashhad University of Medical Sciences, Mashhad, Iran.
Zhong Xi Yi Jie He Xue Bao. 2011 Oct;9(10):1118-24. doi: 10.3736/jcim20111013.
In the present study, the effects of aqueous, ethanolic and chloroformic extracts of Rosa damascena on pentylenetetrazol (PTZ)-induced seizures were investigated in mice.
The animals were divided into the following groups: normal saline control group, diazepam group (3 mg/kg), three aqueous extract groups (100, 500 and 1 000 mg/kg), three ethanolic extract groups (100, 500 and 1 000 mg/kg) and three chloroformic extract groups (100, 500 and 1 000 mg/kg). The extracts, normal saline or diazepam were injected intraperitoneally 30 min before PTZ injection. Latency to the first minimal clonic seizure (MCS) and generalized tonic-clonic seizure (GTCS) and the percent of mortality of rats in each group were recorded.
Significant increases in both MCS and GTCS latencies were observed in all the three aqueous extract groups in comparison with the normal saline control group (P<0.05 or P<0.01). The MCS latency in the ethanolic extract (1 000 mg/kg) group and the GTCS latencies in the two ethanolic extract (500 and 1 000 mg/kg) groups were higher than those in the normal saline control group (P<0.05, P<0.01). There were no significant differences in MCS and GTCS latencies between the three chloroformic extract groups and the normal saline control group. No significant differences were seen in mortality rate following PTZ administration between the different extracts-treated mice and the control mice.
The results of the present study showed that R. damascena has an anticonvulsant effect in a mouse model of PTZ-induced seizures but the exact mechanism of this effect should be clarified in future studies.
在本研究中，考察了大马士革蔷薇水提取物、乙醇提取物和氯仿提取物对小鼠戊四氮（PTZ）诱导癫痫发作的影响。
将动物分为以下几组：生理盐水对照组、地西泮组（3mg/kg）、三个水提取物组（100、500和1000mg/kg）、三个乙醇提取物组（100、500和1000mg/kg）以及三个氯仿提取物组（100、500和1000mg/kg）。在注射PTZ前30分钟腹腔注射提取物、生理盐水或地西泮。记录每组大鼠首次出现最小阵挛性发作（MCS）和全身性强直-阵挛性发作（GTCS）的潜伏期以及死亡率。
与生理盐水对照组相比，所有三个水提取物组的MCS和GTCS潜伏期均显著延长（P<0.05或P<0.01）。乙醇提取物（1000mg/kg）组的MCS潜伏期以及两个乙醇提取物（500和1000mg/kg）组的GTCS潜伏期均高于生理盐水对照组（P<0.05，P<0.01）。三个氯仿提取物组与生理盐水对照组之间的MCS和GTCS潜伏期无显著差异。不同提取物处理的小鼠与对照小鼠在注射PTZ后的死亡率无显著差异。
本研究结果表明，大马士革蔷薇在PTZ诱导的小鼠癫痫发作模型中具有抗惊厥作用，但这种作用的确切机制有待未来研究阐明。
