College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
Mol Cell Biochem. 2012 Mar;362(1-2):87-91. doi: 10.1007/s11010-011-1130-9. Epub 2011 Oct 21.
The major role of insulin-like growth factor-1 (IGF-1) in the liver is to mediate glucose uptake in hepatocytes to synthesize glycogen and maintain blood glucose homeostasis. In this study, to evaluate the role of IGF-1 on gluconeogenesis and nutrient metabolism in dairy cattle, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK) expression and enzyme activity were evaluated in primary cultures of bovine hepatocytes treated with different concentrations of IGF-1 by quantitative polymerase chain reaction and spectrophotometry, respectively. The results showed that expression of PC and PEPCK were significantly lower in bovine hepatocytes by IGF-1 treatment in test group compare to the control group (P < 0.01). As IGF-1 concentration increased, PC and PEPCK enzyme activity in bovine hepatocytes decreased. Evaluating PC and PEPCK mRNA levels and enzyme activity may thus be useful to monitor subclinical ketosis in dairy cows.
胰岛素样生长因子-1（IGF-1）在肝脏中的主要作用是介导肝细胞摄取葡萄糖以合成糖原并维持血糖稳态。在这项研究中，为了评估 IGF-1 在奶牛糖异生和营养代谢中的作用，通过定量聚合酶链反应和分光光度法分别评估了不同浓度 IGF-1 处理的牛原代肝细胞中丙酮酸羧化酶（PC）和磷酸烯醇丙酮酸羧激酶（PEPCK）的表达和酶活性。结果表明，与对照组相比，试验组 IGF-1 处理后牛肝细胞中 PC 和 PEPCK 的表达显著降低（P<0.01）。随着 IGF-1 浓度的增加，牛肝细胞中 PC 和 PEPCK 的酶活性降低。因此，评估 PC 和 PEPCK 的 mRNA 水平和酶活性可能有助于监测奶牛亚临床酮病。
