耐甲氧西林表皮葡萄球菌携带生物膜形成基因：多重 PCR 检测临床分离株。
Methicillin-resistant Staphylococcus epidermidis carrying biofilm formation genes: detection of clinical isolates by multiplex PCR.
机构信息
Laboratory of Hospital Infections, Department of Medical Microbiology, Institute of Microbiology, Rio de Janeiro Federal University, Rio de Janeiro, Brazil.
出版信息
Int Microbiol. 2011 Mar;14(1):13-7. doi: 10.2436/20.1501.01.130.
Staphylococcus epidermidis is the most prevalent coagulase-negative Staphylococcus (CNS) and is a major cause of hospital bacteremia. Based on 18 reference strains and 149 Staphylococcus clinical strains, used in a novel multiplex PCR method, the aim of this study was to identify S. epidermidis with respect to the sequence of three genes: recN, which encodes a recombination/repair protein, mecA (methicillin resistance), and icaAB, which is involved in biofilm formation. Amplicons of 219 bp (S. epidermidis-recN gene), 154 bp (mecA gene), and 546 bp (icaAB genes) were obtained. Reliable results were achieved for 100% of the evaluated strains, suggesting that this new multiplex-PCR approach could be useful for the accurate identification of methicillin-resistant S. epidermidis with the potential to produce biofilm.
表皮葡萄球菌是最常见的凝固酶阴性葡萄球菌（CNS），也是医院菌血症的主要原因。本研究基于一种新的多重 PCR 方法，使用了 18 个参考菌株和 149 个葡萄球菌临床菌株，旨在根据三个基因的序列来鉴定表皮葡萄球菌：recN 基因，该基因编码重组/修复蛋白；mecA（耐甲氧西林）基因；icaAB 基因，该基因为生物膜形成所涉及。获得了 219 bp（表皮葡萄球菌-recN 基因）、154 bp（mecA 基因）和 546 bp（icaAB 基因）的扩增子。评估的菌株 100%都获得了可靠的结果，表明这种新的多重 PCR 方法可用于准确鉴定具有生物膜形成能力的耐甲氧西林表皮葡萄球菌。
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