Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Dev Dyn. 2011 Sep;240(9):2221-31. doi: 10.1002/dvdy.22710.
In contrast to mammals, the brain of the adult zebrafish has a remarkable ability to regenerate. In mammals, injuries induce proliferation of astrocytes and oligodendrocyte progenitors contributing to the formation of a glial scar. We analyzed the proliferation of glial cells and microglia in response to stab injury in the adult zebrafish telencephalon: Radial glial markers were up-regulated at the ventricle and co-expressed the proliferation nuclear antigen (PCNA). Microglia and oligodendrocyte progenitors accumulated transiently at the site of lesion. However, we could not find evidence of permanent scar formation. Parenchymal proliferation was almost negligible in comparison to the increase in proliferation at the ventricular zone. This suggests that most of the cellular material for regeneration is derived from regions of constitutive neurogenesis. Remarkably, the proliferative response is almost completely restricted to the lesioned hemisphere indicating that signals inducing regeneration remain mainly confined within the lesioned half of the telencephalon.
与哺乳动物相反，成年斑马鱼的大脑具有显著的再生能力。在哺乳动物中，损伤会诱导星形胶质细胞和少突胶质细胞前体的增殖，从而形成胶质瘢痕。我们分析了成年斑马鱼端脑受到刺伤后的神经胶质细胞和小胶质细胞的增殖情况：室管膜下区的放射状胶质细胞标志物上调，并共同表达增殖核抗原（PCNA）。小胶质细胞和少突胶质细胞前体在损伤部位短暂积累。然而，我们没有发现永久性瘢痕形成的证据。与室管膜下区的增殖相比，实质细胞的增殖几乎可以忽略不计。这表明，再生所需的大部分细胞物质都来自于固有神经发生的区域。值得注意的是，增殖反应几乎完全局限于损伤的半球，这表明诱导再生的信号主要局限在大脑损伤的一半。
