Marzo A, Cardace G, Monti N, Muck S, Arrigoni Martelli E
Sigma Tau S.p.A., Research and Development Division, Rome, Italy.
J Chromatogr. 1990 May 18;527(2):247-58. doi: 10.1016/s0378-4347(00)82109-x.
L-Carnitine and its acyl esters constitute an endogenous pool of the L-carnitine family, involved in the uptake of free fatty acids in the mitochondria by transfer across their membrane of the acyl moieties to fuel the beta-oxidation and the release of the acetyl group from the mitochondria to the cytosol. Therefore acyl-L-carnitine and acyl-L-carnitine transferase are involved in a homeostatic equilibrium with the cells. As most of these substances need to be monitored in foods, chemical and pharmaceutical processes and biological fluids, an overview of the main methods for assaying them is provided here, with specific reference to the intrinsic performance of each analytical procedure and with suggestions on the correct storage and manipulation of analytical samples.
左旋肉碱及其酰基酯构成了左旋肉碱家族的内源性储备池，通过将酰基部分跨膜转运参与线粒体中游离脂肪酸的摄取，为β-氧化提供燃料，并将乙酰基从线粒体释放到细胞质中。因此，酰基左旋肉碱和酰基左旋肉碱转移酶参与细胞的稳态平衡。由于这些物质中的大多数需要在食品、化学和制药过程以及生物流体中进行监测，本文提供了检测它们的主要方法概述，特别提及了每种分析方法的内在性能，并对分析样品的正确储存和处理提出了建议。
