[采用HPLC-ELSD内标法测定黄芪八个部位中黄芪甲苷的含量]
[Determination of astragaloside IV of eight area in Astragali Radix is by HPLC-ELSD internal standard method].
作者信息
Pei Caiyun, Wang Zongquan, Ja Jiming, Song Jian
机构信息
Hebei Yiling Medicine Institute, Shijiazhuang 050035, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1982-4.
OBJECTIVE
To establish an HPLC-ELSD internal standard method for determination of astragaloside IV in Astragali Radix.
METHOD
With Ginsenoside Rb2 as internal standard, the separation were carried out on an Agilent TC-C18 (4.6 mm x 150 mm, 3.5 microm) column with methanol-water (72: 28) as mobile phase. The flow rate was 1.0 mL x min(-1) and the drift tube temperature of the ELSD was 75 degrees C. The gas pressure was set at 172.4 kPa using the clean and dry compressed air as spray gas.
RESULT
There was good linearity in the range of 0.5624-5.624 microg of astragaloside IV (r = 0.9999); The average recovery was 98.06% with RSD of 0. 98%.
CONCLUSION
The internal standard method is accurate and reproducible, and suitable for quality control of radix astragali.
目的
建立HPLC-ELSD内标法测定黄芪中黄芪甲苷的含量。
方法
以人参皂苷Rb2为内标，采用Agilent TC-C18（4.6 mm×150 mm，3.5 µm）色谱柱，以甲醇-水（72:28）为流动相进行分离。流速为1.0 mL·min-1，蒸发光散射检测器（ELSD）漂移管温度为75℃。以干燥洁净的压缩空气为喷雾气，气体压力设定为172.4 kPa。
结果
黄芪甲苷在0.5624～5.624 μg范围内线性关系良好（r = 0.9999）；平均回收率为98.06%，相对标准偏差（RSD）为0.98%。
结论
该内标法准确、重现性好，适用于黄芪的质量控制。
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