窄叶鲜卑花对高脂血症大鼠脂质代谢的影响及机制
[Effects and mechanism of Sibiraea angustata on lipid metabolism in hight-fatted rats].
作者信息
Xia Miao, Wang Jing-yu, Ma Xiao-yan, Lu Yan-song, Tian Qing-shan, Liu Xin
机构信息
Department of Pathology and Pathophysiology, Basic Medical Science of Lanzhou University, Lanzhou 730000, China.
出版信息
Zhong Yao Cai. 2011 Jun;34(6):922-6.
OBJECTIVE
To investigate the effect and mechanism of Sibiraea angustata on lipid metabolism in hight-fatted SD rats.
METHODS
After the obese model was built,Sibiraea angustata was administrated intragastrically to obese rats for 8 weeks. Peeled off fat around kidneys and made pathological tissue sections. The number and size of adipocytes were detected. The levels of adiponectin, adipoR2, AMPK, and PPARgamma mRNA in adipose tissue were detected by RT-PCR. AMPK protein expression in adipose tissue were detected by Western Blot.
RESULTS
Compared with the model group, the diameter of adipocytes were reduced while the number increased after adminiseration of Sibiraea angustata for 8 weeks. The levels of adiponectin, adipoR2, AMPK and PPARgamma mRNA were increased siginficantly. The expression of AMPK protein was also up-regulated significantly.
CONCLUSION
Sibiraea angustata has anti-obesity effect. The mechanism may be related to the adiponectin signal transduction pathway.
目的
探讨窄叶鲜卑花对高脂SD大鼠脂质代谢的影响及机制。
方法
建立肥胖模型后，对肥胖大鼠灌胃给予窄叶鲜卑花8周。剥离肾脏周围脂肪并制作病理组织切片。检测脂肪细胞数量和大小。采用RT-PCR检测脂肪组织中脂联素、脂联素受体2（adipoR2）、腺苷酸活化蛋白激酶（AMPK）和过氧化物酶体增殖物激活受体γ（PPARγ）mRNA水平。采用蛋白质免疫印迹法检测脂肪组织中AMPK蛋白表达。
结果
与模型组比较，给予窄叶鲜卑花8周后，脂肪细胞直径减小而数量增加。脂联素、adipoR2、AMPK和PPARγ mRNA水平显著升高。AMPK蛋白表达也显著上调。
结论
窄叶鲜卑花具有抗肥胖作用。其机制可能与脂联素信号转导通路有关。
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