He Xiu-min, Yang Qin-he, Li Pei-fang, Chen Zhong, Feng Gao-fei, Wang Wen-jing, Liu Hai-tao, Zhang Yu-pei, Ji Gui-yuan
Department of Traditional Chinese Medicine, Medical College of Jinan University, Guangzhou 510632, China.
Zhong Yao Cai. 2011 Jun;34(6):931-7.
To investigate the effects of soothing liver and invigorating spleen recipes on expression levels of Sterol Regulatory Element-binding Protein-1c (SREBP-1c) mRNA and SREBP-1c protein in hepatic tissue of rats with non-alcoholic fatty liver disease (NAFLD).
Fifty-five SD rats were randomized into 5 groups: normal control group, model group, soothing liver group, invigorating spleen group and combination group. Except the normal group, the rats in model group and other treatment groups were fed with high-fat emulsion to induce NAFLD. The treatment groups were administered with respective traditional chinese medicine, the normal group and model group received correspondence volume distilled water. After treatment for 8 weeks, the rats were executed to obtain the liver for observing hepatic pathological changes. Expression levels of SREBP-1c mRNA and SREBP-1c protein in hepatic tissues were assayed using reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry respectively.
Compared with the normal group, the expression level of SREBP-1c mRNA and SREBP-1c protein in rat hepatic tissues of model group was significantly increased (P<0.01). Compared with the model group,the expression levels of SREBP-1c mRNA and SREBP-lc protein in the treated groups was decreased (P<0.01, P<0.05). Specially, expression levels of SREBP-1c mRNA were the lowest in soothing liver group and invigorating spleen group and hepatic fatty changes were the slightest.
Soothing liver and invigorating spleen recipes can inhibit hepatic SREBP-1c expression. That may be one of their therapeutic mechanisms.
探讨疏肝健脾方对非酒精性脂肪性肝病（NAFLD）大鼠肝组织中固醇调节元件结合蛋白-1c（SREBP-1c）mRNA及SREBP-1c蛋白表达水平的影响。
将55只SD大鼠随机分为5组：正常对照组、模型组、疏肝组、健脾组和联合组。除正常组外，模型组及其他治疗组大鼠均喂以高脂乳剂诱导NAFLD。各治疗组给予相应的中药，正常组和模型组给予等量蒸馏水。治疗8周后处死大鼠，取肝脏观察肝组织病理变化。分别采用逆转录聚合酶链反应（RT-PCR）和免疫组化法检测肝组织中SREBP-1c mRNA及SREBP-1c蛋白的表达水平。
与正常组比较，模型组大鼠肝组织中SREBP-1c mRNA及SREBP-1c蛋白表达水平显著升高（P<0.01）。与模型组比较，各治疗组SREBP-1c mRNA及SREBP-1c蛋白表达水平降低（P<0.01，P<0.05）。其中，疏肝组和健脾组SREBP-1c mRNA表达水平最低，肝脏脂肪变最轻。
疏肝健脾方可抑制肝脏SREBP-1c表达，这可能是其治疗机制之一。
