Shen Hong, Zhu Ling-ying, Yao Nan, Wu Jie
Jiangsu Provincial Insititute of Traditional Chinese Medicine, Jiangsu Research Center for Modern TCM Pharmaceutics Process, Nanjing 210028, China.
Zhong Yao Cai. 2011 Jun;34(6):937-42.
To established an HPLC-MS assay to determine aconitine, mesaconitine and hypaconitine simultaneously in rat plasma and be used to investigate the pharmacokinetics of the three alkaloids.
Three groups of rats were orally administered respectively with three decoctions including decoction of Radix Aconiti Laterlis, blend decoction of Radix Aconiti Laterlis and Radix Glycyrrhizae which decocted separately, decoction of Radix Aconiti Laterlis and Radix Glycyrrhizae which decocted together,the dosage of Radix Aconiti Laterlis was 1.5 g/kg. The contents of aconitine, mesaconitine and hypaconitine in rat plasma were detected using a liquid Chromatography-electrospray ionization/tandem mass spectrometry method. Pharmacokinetic parameters were estimated using DAS 2.0.
The pharmacokinetic parameters of the three compounds obtained showed that Cmax and AUC of aconitine, mesaconitine and hypaconitine were decreased. MRT, t1/2 were prolonged and there was no obviously change in Tmax when Radix Aconiti Lateralis was combined with Radix Glycyrrhizae. The effect of decoction which decocted together was more prominent than which decocted separately.
There are obvious effects on pharmacokinetic of Aconitine, Mesaconitine and Hypaconitine in Rat Plasma when Radix Aconiti Laterlis is combined with Radix Glycyrrhizae.
建立一种高效液相色谱-质谱联用法（HPLC-MS），用于同时测定大鼠血浆中乌头碱、中乌头碱和次乌头碱，并用于研究这三种生物碱的药代动力学。
将三组大鼠分别口服给予三种汤剂，包括单独煎煮的附子汤、附子与甘草分别煎煮的混合汤剂、附子与甘草一起煎煮的汤剂，附子剂量为1.5 g/kg。采用液相色谱-电喷雾电离/串联质谱法检测大鼠血浆中乌头碱、中乌头碱和次乌头碱的含量。使用DAS 2.0估算药代动力学参数。
所得三种化合物的药代动力学参数表明，附子与甘草配伍时，乌头碱、中乌头碱和次乌头碱的Cmax和AUC降低，MRT、t1/2延长，Tmax无明显变化。一起煎煮的汤剂效果比分别煎煮的更显著。
附子与甘草配伍对大鼠血浆中乌头碱、中乌头碱和次乌头碱的药代动力学有明显影响。
