Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Cell Immunol. 2011;272(1):71-8. doi: 10.1016/j.cellimm.2011.09.005. Epub 2011 Sep 22.
Natural killer (NK) cells are important effectors in resistance to viral infections. The role of NK cells in the acute response to human immunodeficiency virus 1 (HIV-1) infected cells was investigated in a mouse model based on a HIV-1/murine leukemia virus (MuLV) pseudovirus. Splenocytes infected with HIV-1/MuLV were injected intraperitoneally and local immunologic responses and persistence of infected cells were investigated. In vivo depletion with an anti-NK1.1 antibody showed that NK cells are important in resistance to virus infected cells. Moreover, NK cell frequency in the peritoneal cavity increased in response to infected cells and these NK cells had a more mature phenotype, as determined by CD27 and Mac-1 expression. Interestingly, after injection of HIV-1/MuLV infected cells, but not MuLV infected cells, peritoneal NK cells had an increased cytotoxic activity. In conclusion, NK cells play a role in the early control of HIV-1/MuLV infected cells in vivo.
自然杀伤 (NK) 细胞是抵抗病毒感染的重要效应器。本研究通过一种基于 HIV-1/鼠白血病病毒 (MuLV) 假病毒的小鼠模型，探究了 NK 细胞在人类免疫缺陷病毒 1 (HIV-1) 感染细胞的急性反应中的作用。将感染 HIV-1/MuLV 的脾细胞经腹腔内注射，并研究局部免疫反应和感染细胞的持续存在情况。用抗 NK1.1 抗体进行体内耗竭实验表明，NK 细胞在抵抗病毒感染细胞方面发挥着重要作用。此外，腹腔内 NK 细胞的频率会因感染细胞而增加，并且这些 NK 细胞的表型更为成熟，表现在 CD27 和 Mac-1 的表达上。有趣的是，在注射感染 HIV-1/MuLV 的细胞后，而非 MuLV 感染的细胞后，腹腔 NK 细胞的细胞毒性活性增强。综上所述，NK 细胞在体内对 HIV-1/MuLV 感染细胞的早期控制中发挥作用。
