SARL BIOEXIGENCE, Besançon, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 15;879(30):3599-606. doi: 10.1016/j.jchromb.2011.09.052. Epub 2011 Oct 6.
A simple, highly selective, sensitive and reproducible liquid chromatography-electrospray ionization mass spectrometry method has been developed for the direct and simultaneous determination of reduced (GSH) and oxidized (GSSG) glutathione in microdialysis samples from human dermis. Chromatographic separation was carried out on an MODULO CART QS KROMASIL 5C18 (250 mm × 2 mm × 5 μm) analytical column at a flow rate of 0.25 ml/min. An isocratic mode was used and consisted of acidified water and acetonitrile (50/50, v/v). To improve the sensitivity, silver nitrate was added as post-column reagent. A trap mass spectrum was used equipped with an ESI interface. The limits of detection and quantification were respectively 0.12 and 0.4 ng/ml for GSH and 0.2 and 0.5 ng/ml for GSSG. Intra-day and inter-day accuracy and precision were determined and the variability was less than 6.2% (R.S.D.).
已经开发出一种简单、高选择性、灵敏且可重现的液相色谱-电喷雾电离质谱法，用于直接和同时测定人真皮微透析样品中的还原型（GSH）和氧化型（GSSG）谷胱甘肽。在 MODULO CART QS KROMASIL 5C18（250mm×2mm×5μm）分析柱上以 0.25ml/min 的流速进行色谱分离。采用等度模式，由酸化水和乙腈（50/50，v/v）组成。为了提高灵敏度，在柱后添加硝酸银作为试剂。使用带有 ESI 接口的阱质谱进行分析。GSH 的检测限和定量限分别为 0.12 和 0.4ng/ml，GSSG 的检测限和定量限分别为 0.2 和 0.5ng/ml。测定了日内和日间的准确性和精密度，变异性小于 6.2%（RSD）。
