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A novel series of pEX-PINK expression vectors for screening high-level production of (un)fused foreign proteins by rapid visual detection of PINK Escherichia coli clones.

作者信息

Kaderbhai M A, Sligar S G, Barnfield T, Reames T, Gallagher J, He M, Mercer E I, Kaderbhai N

机构信息

Department of Biochemistry, University College of Wales, Penglais, Aberystwyth, UK.

出版信息

Nucleic Acids Res. 1990 Aug 11;18(15):4629-30. doi: 10.1093/nar/18.15.4629.

DOI:10.1093/nar/18.15.4629
PMID:2201954
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331320/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6a/331320/cd436923efef/nar00199-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6a/331320/cd436923efef/nar00199-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6a/331320/cd436923efef/nar00199-0309-a.jpg

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Export of a hyperexpressed mammalian globular cytochrome b5 precursor in Escherichia coli is dramatically affected by the nature of the amino acid flanking the secretory signal sequence cleavage bond.在大肠杆菌中表达的哺乳动物球状细胞色素 b5 前体的分泌受到紧邻信号序列切割位点的氨基酸性质的显著影响。
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A directed evolution strategy for optimized export of recombinant proteins reveals critical determinants for preprotein discharge.一种用于优化重组蛋白输出的定向进化策略揭示了前体蛋白排出的关键决定因素。
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本文引用的文献

1
Synthesis, bacterial expression, and mutagenesis of the gene coding for mammalian cytochrome b5.哺乳动物细胞色素b5编码基因的合成、细菌表达及诱变
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9443-7. doi: 10.1073/pnas.83.24.9443.
2
Versatile expression vectors for high-level synthesis of cloned gene products in Escherichia coli.用于在大肠杆菌中高水平合成克隆基因产物的多功能表达载体。
Gene. 1985;38(1-3):31-8. doi: 10.1016/0378-1119(85)90200-8.
3
Co-expression of a precursor and the mature protein of wheat ribulose-1,5-bisphosphate carboxylase small subunit from a single gene in Escherichia coli.
Regulation of heme biosynthesis in Salmonella typhimurium: activity of glutamyl-tRNA reductase (HemA) is greatly elevated during heme limitation by a mechanism which increases abundance of the protein.
鼠伤寒沙门氏菌中血红素生物合成的调控：在血红素受限期间，谷氨酰 - tRNA还原酶（HemA）的活性通过增加蛋白质丰度的机制大幅升高。
J Bacteriol. 1997 May;179(9):2907-14. doi: 10.1128/jb.179.9.2907-2914.1997.
从小麦核酮糖-1,5-二磷酸羧化酶小亚基的单个基因在大肠杆菌中同时表达前体和成熟蛋白。
DNA Cell Biol. 1990 Jan-Feb;9(1):11-25. doi: 10.1089/dna.1990.9.11.