IVI Barcelona, Ronda General Mitre 14, 08017 Barcelona, Spain.
Reprod Biomed Online. 2011 Dec;23(6):704-10. doi: 10.1016/j.rbmo.2011.07.010. Epub 2011 Jul 22.
A prospective study was performed to assess the impact of sperm DNA fragmentation on the outcome of IVF with own or donated oocytes. The study population included 178 couples (62 cycles of IVF, 116 of intracytoplasmic sperm injection (ICSI)) with own (n=77) and donor (n=101) oocytes. DNA fragmentation was evaluated by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay. Correlation between DNA damage to oocyte fertilization, embryo quality and clinical pregnancy, implantation and miscarriage rates was evaluated. DNA fragmentation was not related to fertilization rates in either IVF (r=0.08) or ICSI (r=-0.04) cycles. DNA fragmentation was similar in patients with <50% embryo utilization rate compared with ≥50%, in cancelled and in embryo transfer cycles and in miscarriages and in successful deliveries. Moreover, DNA fragmentation was similar in pregnant and non-pregnant women as well as in IVF with own or donor oocytes. In the multivariable analysis, the odds ratio of DNA after controlling by age was 1.0. Using a 36% sperm fragmentation threshold, results did not vary. It is concluded that DNA damage was not related to outcomes of IVF or ICSI with own or donor oocytes.
一项前瞻性研究旨在评估精子 DNA 碎片化对自身或捐赠卵子体外受精（IVF）结局的影响。研究人群包括 178 对夫妇（62 个 IVF 周期，116 个胞浆内单精子注射（ICSI）周期），包括自身（n=77）和供体（n=101）卵子。通过末端脱氧核苷酸转移酶（TdT）介导的 dUDP 末端标记法评估 DNA 碎片化。评估了 DNA 损伤与卵子受精、胚胎质量以及临床妊娠、着床和流产率之间的相关性。DNA 碎片化与 IVF（r=0.08）或 ICSI（r=-0.04）周期的受精率均无相关性。在胚胎利用率<50%与≥50%、取消周期和胚胎移植周期、流产和成功分娩的患者中，DNA 碎片化相似。此外，在妊娠和非妊娠妇女中，以及在自身或供体卵子的 IVF 中，DNA 碎片化也相似。在多变量分析中，在控制年龄后，DNA 的优势比为 1.0。使用 36%的精子碎片化阈值，结果没有变化。因此，结论是 DNA 损伤与自身或供体卵子的 IVF 或 ICSI 结局无关。
