Department of Systems Medicine and Bioengineering, Weill Cornell Medical College of Cornell University, Houston, TX 77030, USA.
J Vasc Interv Radiol. 2011 Dec;22(12):1758-64. doi: 10.1016/j.jvir.2011.08.025. Epub 2011 Oct 22.
To show the feasibility of computed tomography (CT) image-guided fiberoptic confocal fluorescence molecular imaging in a rabbit lung tumor model.
Eight lung tumor models were created by injection of a VX2 cell suspension. The fluorescent imaging agent IntegriSense 680 was given to the animals 3.5-4 hours before the procedure. CT images were obtained and transferred to the minimally invasive multimodality image-guided (MIMIG) system as a guidance map. A real-time electromagnetically tracked needle was inserted under the visual guidance of the MIMIG system. A second CT image was obtained to confirm the location of the needle tip. Next, fiberoptic fluorescence imaging was acquired along the needle track. Finally, tumor samples were obtained for histopathologic confirmation.
All cases were performed during breath-hold. Tumor size was 12.5 mm ± 1.6; the distance from the chest wall was 2.1 mm ± 0.5. The needle tip reached the tumor in all cases with an accuracy of 3.3 mm ± 1.6. Only one skin entry point was necessary, and no needle adjustments were required. No pneumothorax was observed. At least two-fold α(v)β(3) integrin image contrast was detected in the tumor compared with normal lung tissue. Tumor samples were confirmed to have viable VX2 cells and contrast uptake.
The MIMIG system enables effective in situ fluorescence molecular imaging in a needle biopsy lung procedure. In situ α(v)β(3) integrin molecular imaging allows molecular characterization of lung tumors at multiple regions and can be used to guide biopsy procedures.
展示 CT 图像引导光纤共聚焦荧光分子成像在兔肺肿瘤模型中的可行性。
通过注射 VX2 细胞悬液建立 8 个肺肿瘤模型。在手术前 3.5-4 小时给予动物荧光成像剂 IntegriSense 680。获取 CT 图像并传输到微创多模态图像引导（MIMIG）系统作为引导图。在 MIMIG 系统的可视引导下插入实时电磁跟踪针。获取第二张 CT 图像以确认针尖的位置。接下来，沿着针道进行光纤荧光成像。最后，获取肿瘤样本进行组织病理学确认。
所有病例均在屏气时进行。肿瘤大小为 12.5mm±1.6；距胸壁的距离为 2.1mm±0.5。所有病例的针尖均到达肿瘤，准确性为 3.3mm±1.6。仅需一个皮肤入路，无需调整针。未观察到气胸。与正常肺组织相比，肿瘤中至少检测到两倍的α(v)β(3)整合素图像对比度。肿瘤样本被证实含有活的 VX2 细胞和对比剂摄取。
MIMIG 系统可在经皮肺活检过程中实现有效的原位荧光分子成像。原位α(v)β(3)整合素分子成像可对多个区域的肺肿瘤进行分子特征描述，并可用于指导活检程序。
