Taniguchi M, Arikawa R, Kaedei Y, Tanihara F, Namula Z, Viet V L, Sato Y, Otoi T
Department of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
Cryo Letters. 2011 Sep-Oct;32(5):410-4.
Chemical toxicity of cryoprotectants to in vitro developmental competence of porcine oocytes was examined. In vitro-matured oocytes were exposed to 40 percent ethylene glycol (EG), glycerol (GLY), or 1,2-propanediol (PD), fertilized with spermatozoa, and cultured for 8 days. Compared to treatment with other cryoprotectants, exposure to EG resulted in the development of significantly more blastocysts, but the rate was significantly lower than that of non-exposed control oocytes. In vitro-matured oocytes were also equilibrated in 40 percent EG by 3 multi-step methods, after which their developmental competence was evaluated. The rate of blastocyst development was higher in the 4-step method than in the 2- and 3-step methods of equilibrium. These results indicate that cryoprotectants and equilibration methods affect the developmental competence of porcine oocytes and that EG may be a superior cryoprotectant for vitrification of these cells.
研究了冷冻保护剂对猪卵母细胞体外发育能力的化学毒性。将体外成熟的卵母细胞暴露于40%的乙二醇（EG）、甘油（GLY）或1,2 - 丙二醇（PD）中，与精子受精，并培养8天。与用其他冷冻保护剂处理相比，暴露于EG导致发育出显著更多的囊胚，但该比率显著低于未暴露的对照卵母细胞。体外成熟的卵母细胞也通过3种多步方法在40%的EG中进行平衡，之后评估其发育能力。4步法的囊胚发育率高于2步和3步平衡法。这些结果表明，冷冻保护剂和平衡方法会影响猪卵母细胞的发育能力，并且EG可能是这些细胞玻璃化冷冻的优质冷冻保护剂。
