两种DNA探针在疟疾诊断中的比较，一种对恶性疟原虫具有特异性，另一种具有更广泛的反应性。

A comparison of two DNA probes, one specific for Plasmodium falciparum and one with wider reactivity, in the diagnosis of malaria.

作者信息

Holmberg M, Vaidya A B, Shenton F C, Snow R W, Greenwood B M, Wigzell H, Pettersson U

机构信息

Department of Immunology, Karolinska Institute, Stockholm, Sweden.

出版信息

Trans R Soc Trop Med Hyg. 1990 Mar-Apr;84(2):202-5. doi: 10.1016/0035-9203(90)90255-d.

DOI:10.1016/0035-9203(90)90255-d

PMID:2202099

Abstract

The sensitivity and specificity of 2 probes for the detection of malarial infection was studied. 399 blood samples from Gambian children were tested in a deoxyribonucleic acid (DNA) hybridization assay, and the results compared with the microscopical findings from thick blood films. 8 additional pure Plasmodium malariae and 14 pure P. vivax samples were also assayed. One probe, containing a 21 base pair tandem repeat and highly specific for P. falciparum, detected this species in all except 2 of 74 samples with a parasitaemia of 250 per microliter or more; the overall sensitivity of the probe was 76%. The other probe, a 6 kilobase pair organelle DNA, is conserved in all Plasmodium species so far tested. Its sensitivity for P. falciparum was lower than the 21 base pair repeat, but it detected P. vivax and P. malariae at low levels of parasitaemia, and thus could be useful in field studies.

摘要

研究了两种用于检测疟疾感染的探针的敏感性和特异性。在一项脱氧核糖核酸（DNA）杂交试验中，对来自冈比亚儿童的399份血样进行了检测，并将结果与厚血膜显微镜检查结果进行了比较。另外还检测了8份纯恶性疟原虫样本和14份纯间日疟原虫样本。一种探针含有21个碱基对的串联重复序列，对恶性疟原虫具有高度特异性，在每微升250个或更多疟原虫血症的74份样本中，除2份外均检测到了该物种；该探针的总体敏感性为76%。另一种探针是一个6千碱基对的细胞器DNA，在迄今为止测试的所有疟原虫物种中都保守。它对恶性疟原虫的敏感性低于21个碱基对重复序列，但它能在低疟原虫血症水平下检测到间日疟原虫和三日疟原虫，因此可用于现场研究。