State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Fudan University, Shanghai, China.
Cereb Cortex. 2012 Sep;22(9):2120-30. doi: 10.1093/cercor/bhr296. Epub 2011 Oct 20.
Cortical GABAergic interneurons in rodents originate from subpallial progenitors and tangentially migrate to the cortex. While the majority of mouse neocortical interneurons are derived from the medial and caudal ganglionic eminence (MGE and CGE, respectively), it remains unknown whether the lateral ganglionic eminence (LGE) also contributes to a subpopulation of cortical interneurons. Here, we show that the transcription factor Sp8 is expressed in one-fifth of adult cortical interneurons, which appear to be derived from both the dorsal LGE and the dorsal CGE (dLGE and dCGE, respectively). Compared with the MGE-derived cortical interneurons, dLGE/dCGE-derived Sp8-expressing (Sp8+) ones are born at later embryonic stages with peak production occurring at embryonic day 15.5. They tangentially migrate mainly along the subventricular/intermediate zone (SVZ/IZ) route; some continue to express mitotic markers (Ki67 and PH3) in the neonatal cortical SVZ/IZ. Sp8+ interneurons continue to radially migrate from the SVZ/IZ into the cortical layers at early postnatal stages. In contrast to MGE-derived interneurons, dLGE/dCGE-derived Sp8+ interneurons follow an outside-in layering pattern, preferentially occupying superficial cortical layers.
啮齿动物大脑皮层 GABA 能中间神经元起源于皮层下祖细胞，并经切向迁移到皮层。虽然大多数小鼠新皮层中间神经元来源于内侧和尾状神经节隆起（MGE 和 CGE，分别），但 lateral ganglionic eminence（LGE）是否也有助于皮质中间神经元的亚群尚不清楚。在这里，我们表明转录因子 Sp8 在五分之一的成年皮质中间神经元中表达，这些细胞似乎来源于背侧 LGE 和背侧 CGE（分别为 dLGE 和 dCGE）。与 MGE 衍生的皮质中间神经元相比，dLGE/dCGE 衍生的 Sp8 表达（Sp8+）细胞在胚胎期晚期出生，出生高峰期发生在胚胎期 15.5 天。它们主要沿侧脑室/中间区（SVZ/IZ）途径进行切向迁移；一些在新生儿皮质 SVZ/IZ 中继续表达有丝分裂标志物（Ki67 和 PH3）。Sp8+中间神经元在出生后早期继续从 SVZ/IZ 径向迁移到皮质层。与 MGE 衍生的中间神经元不同，dLGE/dCGE 衍生的 Sp8+中间神经元遵循从外向内的分层模式，优先占据浅层皮质层。
